Abdelazeem M. Algammal, Mahmoud Mabrok, Khyreyah J. Alfifi, Saad Alghamdi, Dalia M. Alammari, Madeha O. I. Ghobashy, Mohammad Y. Alshahrani, Azza S. El-Demerdash, El-Sayed Hemdan Eissa, Aya M. Elalamy, Reham M. El-Tarabili
{"title":"在海鳟中不断进化的多重耐药溶藻弧菌通常含有胶原酶、trh和tlh毒力基因以及sul1、blaTEM、aadA、tetA、blaOXA和tetB或tetM耐药基因","authors":"Abdelazeem M. Algammal, Mahmoud Mabrok, Khyreyah J. Alfifi, Saad Alghamdi, Dalia M. Alammari, Madeha O. I. Ghobashy, Mohammad Y. Alshahrani, Azza S. El-Demerdash, El-Sayed Hemdan Eissa, Aya M. Elalamy, Reham M. El-Tarabili","doi":"10.1007/s10499-024-01812-w","DOIUrl":null,"url":null,"abstract":"<div><p><i>Vibrio alginolyticus</i> is associated with severe infections in marine fish and immunocompromised people. This study investigated the prevalence, genetic typing, associated virulence traits, unveiling resistance genes, and the pathogenicity of <i>V. alginolyticus</i> isolated from sea bream. A total of 200 sea bream (<i>Sparus aurata</i>) were gathered from secluded farms in Port Said, Egypt. Subsequently, clinical, postmortem, and bacteriological analyses, including morphological, culture, biochemical characterization, and PCR-based collagenase gene detection, were performed. The isolated strains were tested for <i>collagenase</i> gene sequencing, antibiogram, pathogenicity, and PCR monitoring of virulence and resistance genes. Herein, <i>V. alginolyticus</i> was found in 14% (28/200) of the collected sea bream, exclusively from the moribund fish. Moreover, the collagenase phylogeny revealed that the tested<i> V. alginolyticus</i> isolates have a distinguished genetic homogeneity with other <i>V. alginolyticus</i> isolates from China, Japan, and the USA. Moreover, the most predominant virulence genes in the tested <i>V. alginolyticus</i> were the <i>collagenase</i> (100%), <i>trh</i> (90.4%), <i>tlh</i> (59.6%), and <i>tdh</i> (21.1%) genes. In this study, 28.8% of the obtained <i>V. alginolyticus</i> strains were multidrug-resistant (MDR) to five classes and encoded <i>bla</i><sub>TEM</sub>,<i> aad</i>A, <i>tet</i>A, <i>bla</i><sub>OXA</sub>, and<i> sul</i>1 genes. Likewise, 15.4% of <i>V</i>. <i>alginolyticus</i> were MDR to four classes and encoded <i>sul</i>1 and <i>tet</i>B genes. As well, 7.7% were MDR to six classes and encoded <i>bla</i><sub>TEM</sub>,<i> tet</i>M, <i>sul</i>1, and <i>bla</i><sub>OXA</sub> genes. Using the cumulative mortality curve, a high mortality rate (87%) was recorded within 7 days in the challenged <i>Tilapia zillii</i> with a virulent <i>V. alginolyticus</i> strain. Concisely, this investigation draws attention to the evolution of MDR <i>V. alginolyticus</i> in sea bream, instructing a health problem. The <i>collagenase</i>, <i>trh</i>, and <i>tlh</i> virulence-related genes and the <i>sul</i>1,<i> bla</i><sub>TEM</sub>,<i> aad</i>A,<i> tet</i>A,<i> bla</i><sub>OXA</sub>, and<i> tet</i>B or <i>tet</i>M resistance genes commonly accompanied the evolving MDR <i>V. alginolyticus</i> in sea bream.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 2","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The evolving multidrug-resistant V. alginolyticus in sea bream commonly harbored collagenase, trh, and tlh virulence genes and sul1, blaTEM, aadA, tetA, blaOXA, and tetB or tetM resistance genes\",\"authors\":\"Abdelazeem M. Algammal, Mahmoud Mabrok, Khyreyah J. Alfifi, Saad Alghamdi, Dalia M. Alammari, Madeha O. I. Ghobashy, Mohammad Y. Alshahrani, Azza S. El-Demerdash, El-Sayed Hemdan Eissa, Aya M. Elalamy, Reham M. El-Tarabili\",\"doi\":\"10.1007/s10499-024-01812-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><i>Vibrio alginolyticus</i> is associated with severe infections in marine fish and immunocompromised people. This study investigated the prevalence, genetic typing, associated virulence traits, unveiling resistance genes, and the pathogenicity of <i>V. alginolyticus</i> isolated from sea bream. A total of 200 sea bream (<i>Sparus aurata</i>) were gathered from secluded farms in Port Said, Egypt. Subsequently, clinical, postmortem, and bacteriological analyses, including morphological, culture, biochemical characterization, and PCR-based collagenase gene detection, were performed. The isolated strains were tested for <i>collagenase</i> gene sequencing, antibiogram, pathogenicity, and PCR monitoring of virulence and resistance genes. Herein, <i>V. alginolyticus</i> was found in 14% (28/200) of the collected sea bream, exclusively from the moribund fish. Moreover, the collagenase phylogeny revealed that the tested<i> V. alginolyticus</i> isolates have a distinguished genetic homogeneity with other <i>V. alginolyticus</i> isolates from China, Japan, and the USA. Moreover, the most predominant virulence genes in the tested <i>V. alginolyticus</i> were the <i>collagenase</i> (100%), <i>trh</i> (90.4%), <i>tlh</i> (59.6%), and <i>tdh</i> (21.1%) genes. In this study, 28.8% of the obtained <i>V. alginolyticus</i> strains were multidrug-resistant (MDR) to five classes and encoded <i>bla</i><sub>TEM</sub>,<i> aad</i>A, <i>tet</i>A, <i>bla</i><sub>OXA</sub>, and<i> sul</i>1 genes. Likewise, 15.4% of <i>V</i>. <i>alginolyticus</i> were MDR to four classes and encoded <i>sul</i>1 and <i>tet</i>B genes. As well, 7.7% were MDR to six classes and encoded <i>bla</i><sub>TEM</sub>,<i> tet</i>M, <i>sul</i>1, and <i>bla</i><sub>OXA</sub> genes. Using the cumulative mortality curve, a high mortality rate (87%) was recorded within 7 days in the challenged <i>Tilapia zillii</i> with a virulent <i>V. alginolyticus</i> strain. Concisely, this investigation draws attention to the evolution of MDR <i>V. alginolyticus</i> in sea bream, instructing a health problem. The <i>collagenase</i>, <i>trh</i>, and <i>tlh</i> virulence-related genes and the <i>sul</i>1,<i> bla</i><sub>TEM</sub>,<i> aad</i>A,<i> tet</i>A,<i> bla</i><sub>OXA</sub>, and<i> tet</i>B or <i>tet</i>M resistance genes commonly accompanied the evolving MDR <i>V. alginolyticus</i> in sea bream.</p></div>\",\"PeriodicalId\":8122,\"journal\":{\"name\":\"Aquaculture International\",\"volume\":\"33 2\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-01-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aquaculture International\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10499-024-01812-w\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture International","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s10499-024-01812-w","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FISHERIES","Score":null,"Total":0}
The evolving multidrug-resistant V. alginolyticus in sea bream commonly harbored collagenase, trh, and tlh virulence genes and sul1, blaTEM, aadA, tetA, blaOXA, and tetB or tetM resistance genes
Vibrio alginolyticus is associated with severe infections in marine fish and immunocompromised people. This study investigated the prevalence, genetic typing, associated virulence traits, unveiling resistance genes, and the pathogenicity of V. alginolyticus isolated from sea bream. A total of 200 sea bream (Sparus aurata) were gathered from secluded farms in Port Said, Egypt. Subsequently, clinical, postmortem, and bacteriological analyses, including morphological, culture, biochemical characterization, and PCR-based collagenase gene detection, were performed. The isolated strains were tested for collagenase gene sequencing, antibiogram, pathogenicity, and PCR monitoring of virulence and resistance genes. Herein, V. alginolyticus was found in 14% (28/200) of the collected sea bream, exclusively from the moribund fish. Moreover, the collagenase phylogeny revealed that the tested V. alginolyticus isolates have a distinguished genetic homogeneity with other V. alginolyticus isolates from China, Japan, and the USA. Moreover, the most predominant virulence genes in the tested V. alginolyticus were the collagenase (100%), trh (90.4%), tlh (59.6%), and tdh (21.1%) genes. In this study, 28.8% of the obtained V. alginolyticus strains were multidrug-resistant (MDR) to five classes and encoded blaTEM, aadA, tetA, blaOXA, and sul1 genes. Likewise, 15.4% of V. alginolyticus were MDR to four classes and encoded sul1 and tetB genes. As well, 7.7% were MDR to six classes and encoded blaTEM, tetM, sul1, and blaOXA genes. Using the cumulative mortality curve, a high mortality rate (87%) was recorded within 7 days in the challenged Tilapia zillii with a virulent V. alginolyticus strain. Concisely, this investigation draws attention to the evolution of MDR V. alginolyticus in sea bream, instructing a health problem. The collagenase, trh, and tlh virulence-related genes and the sul1, blaTEM, aadA, tetA, blaOXA, and tetB or tetM resistance genes commonly accompanied the evolving MDR V. alginolyticus in sea bream.
期刊介绍：
Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture.
The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more.
This is the official Journal of the European Aquaculture Society.
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