Biogenic nanotransferosomal vesicular system of Clerodendrum serratum L. for skin cancer therapy: formulation, characterization, and efficacy evaluation

Background

The pivotal intent of this study was to investigate the medicinal potential of synthesized nanotransferosomes utilizing Clerodendrum serratum L. hydroalcoholic extract (CS-NTs). Three batches, CS-NTs 25, CS-NTs 50, and CS-NTs 100, were formulated using the thin-film hydration approach with varying concentrations of phospholipids (soya phosphatidylcholine) at 25, 50, and 100 mg, respectively, to evaluate their efficacy against skin cancer cell lines. Several analytical approaches, such as UV/Vis spectroscopy, XRD spectra, FTIR, TEM, SEM, particle size distribution, and zeta potential analysis, were employed to assess the synthesized CS-NTs.

Results

Experimental results revealed that CS-NTs exhibited a circular shape, with average vesicle sizes approximately measured at 463.6 ± 100.5 nm, 265.5 ± 61.6 nm, and 409.6 ± 106.2 nm for CS-NTs 25, CS-NTs 50, and CS-NTs 100, respectively. Notably, biologically synthesized CS-NTs 25 offer potential anticancer benefits against B10F16 (skin cancer cell line) cells, exhibiting no signs of adverse effects. The heightened biological potential of CS-NTs can be ascribed to their unique characteristics at the nanoscale, characterized by an average vesicle size and lower polydispersity value, which facilitate augmented responsiveness and interactions with biological systems.

Conclusion

CS-NTs 25 formulated through the biogenic process using C. serratum hydroalcoholic plant root extract hold significant potential for anticancer activity.

Graphical abstract