{"title":"基于增强过氧化物酶样活性的新型Ru-Fe3O4纳米酶灵敏选择性比色法检测甲基硫代盐","authors":"Yiwen Gu, Huixiang Yan, Tianwen Bai, Daoyuan Rao, Yiwen Yang, Hongyu Hu, Lei Li, Longhua Guo, Yanbo Zeng","doi":"10.1007/s00604-024-06907-y","DOIUrl":null,"url":null,"abstract":"<div><p>A novel Ru-Fe<sub>3</sub>O<sub>4</sub> nanozyme with enhanced peroxidase-like (POD-like) activity was synthesized through a hydrothermal method. Ru-Fe<sub>3</sub>O<sub>4</sub> nanozyme was effectively utilized for the detection of thiophanate-methyl (TM) using a colorimetric technique. The POD-like activity of Ru-Fe<sub>3</sub>O<sub>4</sub> was found to be superior compared to Fe<sub>3</sub>O<sub>4</sub>, Rh-Fe<sub>3</sub>O<sub>4</sub>, and Pd-Fe<sub>3</sub>O<sub>4</sub>. Ru-Fe<sub>3</sub>O<sub>4</sub> provided excellent POD-like activity with Michaelis constants of 0.00645 mM and 0.66714 mM for substrates of 3,3’,5,5’-tetramethylbenzidine and H<sub>2</sub>O<sub>2</sub>, respectively. The density functional theory calculation showed that Ru-Fe<sub>3</sub>O<sub>4</sub> had better H<sub>2</sub>O<sub>2</sub> decomposition reactivity compared to Fe<sub>3</sub>O<sub>4</sub>. Compared with Fe<sub>3</sub>O<sub>4</sub>, the adsorbed H<sub>2</sub>O<sub>2</sub> molecules underwent decomposition more readily on the Ru-Fe<sub>3</sub>O<sub>4</sub> catalytic surface facilitating the occurrence of the H<sub>2</sub>O<sub>2</sub> catalytic reaction, further suggesting the excellent POD-like activity of Ru-Fe<sub>3</sub>O<sub>4</sub>. The coordination and electrostatic interactions of Ru-Fe<sub>3</sub>O<sub>4</sub> and TM promoted their binding, contributing to TM covering Ru-Fe<sub>3</sub>O<sub>4</sub> catalytic site and inhibiting the POD-like activity of Ru-Fe<sub>3</sub>O<sub>4</sub>. As a result, the sensitive and selective detection of TM using Ru-Fe<sub>3</sub>O<sub>4</sub> by a colorimetric method was achieved. The Ru-Fe<sub>3</sub>O<sub>4</sub> nanozyme displayed a good linear correlation, with a linear detection range and a detection limit of 0.1–100 and 0.03 μg/mL, respectively. The Ru-Fe<sub>3</sub>O<sub>4</sub> nanozyme was used for the determination of TM in soil and water samples with success.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sensitive and selective colorimetric detection of thiophanate-methyl based on a novel Ru-Fe3O4 nanozyme with enhanced peroxidase-like activity\",\"authors\":\"Yiwen Gu, Huixiang Yan, Tianwen Bai, Daoyuan Rao, Yiwen Yang, Hongyu Hu, Lei Li, Longhua Guo, Yanbo Zeng\",\"doi\":\"10.1007/s00604-024-06907-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A novel Ru-Fe<sub>3</sub>O<sub>4</sub> nanozyme with enhanced peroxidase-like (POD-like) activity was synthesized through a hydrothermal method. Ru-Fe<sub>3</sub>O<sub>4</sub> nanozyme was effectively utilized for the detection of thiophanate-methyl (TM) using a colorimetric technique. The POD-like activity of Ru-Fe<sub>3</sub>O<sub>4</sub> was found to be superior compared to Fe<sub>3</sub>O<sub>4</sub>, Rh-Fe<sub>3</sub>O<sub>4</sub>, and Pd-Fe<sub>3</sub>O<sub>4</sub>. Ru-Fe<sub>3</sub>O<sub>4</sub> provided excellent POD-like activity with Michaelis constants of 0.00645 mM and 0.66714 mM for substrates of 3,3’,5,5’-tetramethylbenzidine and H<sub>2</sub>O<sub>2</sub>, respectively. The density functional theory calculation showed that Ru-Fe<sub>3</sub>O<sub>4</sub> had better H<sub>2</sub>O<sub>2</sub> decomposition reactivity compared to Fe<sub>3</sub>O<sub>4</sub>. Compared with Fe<sub>3</sub>O<sub>4</sub>, the adsorbed H<sub>2</sub>O<sub>2</sub> molecules underwent decomposition more readily on the Ru-Fe<sub>3</sub>O<sub>4</sub> catalytic surface facilitating the occurrence of the H<sub>2</sub>O<sub>2</sub> catalytic reaction, further suggesting the excellent POD-like activity of Ru-Fe<sub>3</sub>O<sub>4</sub>. The coordination and electrostatic interactions of Ru-Fe<sub>3</sub>O<sub>4</sub> and TM promoted their binding, contributing to TM covering Ru-Fe<sub>3</sub>O<sub>4</sub> catalytic site and inhibiting the POD-like activity of Ru-Fe<sub>3</sub>O<sub>4</sub>. As a result, the sensitive and selective detection of TM using Ru-Fe<sub>3</sub>O<sub>4</sub> by a colorimetric method was achieved. The Ru-Fe<sub>3</sub>O<sub>4</sub> nanozyme displayed a good linear correlation, with a linear detection range and a detection limit of 0.1–100 and 0.03 μg/mL, respectively. The Ru-Fe<sub>3</sub>O<sub>4</sub> nanozyme was used for the determination of TM in soil and water samples with success.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 2\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-01-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-024-06907-y\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-024-06907-y","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Sensitive and selective colorimetric detection of thiophanate-methyl based on a novel Ru-Fe3O4 nanozyme with enhanced peroxidase-like activity
A novel Ru-Fe3O4 nanozyme with enhanced peroxidase-like (POD-like) activity was synthesized through a hydrothermal method. Ru-Fe3O4 nanozyme was effectively utilized for the detection of thiophanate-methyl (TM) using a colorimetric technique. The POD-like activity of Ru-Fe3O4 was found to be superior compared to Fe3O4, Rh-Fe3O4, and Pd-Fe3O4. Ru-Fe3O4 provided excellent POD-like activity with Michaelis constants of 0.00645 mM and 0.66714 mM for substrates of 3,3’,5,5’-tetramethylbenzidine and H2O2, respectively. The density functional theory calculation showed that Ru-Fe3O4 had better H2O2 decomposition reactivity compared to Fe3O4. Compared with Fe3O4, the adsorbed H2O2 molecules underwent decomposition more readily on the Ru-Fe3O4 catalytic surface facilitating the occurrence of the H2O2 catalytic reaction, further suggesting the excellent POD-like activity of Ru-Fe3O4. The coordination and electrostatic interactions of Ru-Fe3O4 and TM promoted their binding, contributing to TM covering Ru-Fe3O4 catalytic site and inhibiting the POD-like activity of Ru-Fe3O4. As a result, the sensitive and selective detection of TM using Ru-Fe3O4 by a colorimetric method was achieved. The Ru-Fe3O4 nanozyme displayed a good linear correlation, with a linear detection range and a detection limit of 0.1–100 and 0.03 μg/mL, respectively. The Ru-Fe3O4 nanozyme was used for the determination of TM in soil and water samples with success.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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