敲除睾丸特异性基因簇会损害秋粘虫（Spodoptera frugiperda）的雄性生殖力

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2025-01-07 DOI:10.1002/ps.8634

Pei Wang, Yuchen Zhao, Yalan Cheng, Siyu Tian, Yuxuan Bai, Junfeng Zuo, Subba Reddy Palli, Xien Chen

{"title":"敲除睾丸特异性基因簇会损害秋粘虫（Spodoptera frugiperda）的雄性生殖力","authors":"Pei Wang, Yuchen Zhao, Yalan Cheng, Siyu Tian, Yuxuan Bai, Junfeng Zuo, Subba Reddy Palli, Xien Chen","doi":"10.1002/ps.8634","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> BACKGROUND</h3>\n \n <p>The function of some testis-specific genes (TSGs) in model insects have been studied, but their function in non-model insects remains largely unexplored. In the present study, we identified several TSGs in the fall armyworm (FAW), a significant agricultural pest, through comparative transcriptomic analysis. A testis-specific gene cluster (TSGC) comprising multiple functional genes and long non-coding RNAs was found.</p>\n </section>\n \n <section>\n \n <h3> RESULTS</h3>\n \n <p>Quantitative PCR analysis validated the testis-specific expression of genes within this cluster across different developmental stages. Interspecies collinearity analysis indicated that this TSGC is conserved among lepidopterans. To elucidate the function of this TSGC, we employed the CRISPR/Cas9 gene-editing technique to generate loss-of-function mutants. Notably, the hatching rate of eggs produced by wild-type females mated with mutant males (approximately 30%) was substantially lower than that of eggs from wild-type females mated with wild-type males (approximately 80%). Additionally, a significant reduction in the size and number of sperm was observed in the testis of mutant males compared to wild-type males.</p>\n </section>\n \n <section>\n \n <h3> CONCLUSION</h3>\n \n <p>Collectively, this study not only elucidates the role of the TSGC in supporting male fertility in the FAW, which provides valuable information for understanding the mechanism of male reproduction in insects, but also identifies potential target genes for the development of genetic control strategies against lepidopteran pests. © 2025 Society of Chemical Industry.</p>\n </section>\n </div>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"81 4","pages":"2355-2363"},"PeriodicalIF":3.8000,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Knockout of a testis-specific gene cluster impairs male fertility in the fall armyworm, Spodoptera frugiperda\",\"authors\":\"Pei Wang, Yuchen Zhao, Yalan Cheng, Siyu Tian, Yuxuan Bai, Junfeng Zuo, Subba Reddy Palli, Xien Chen\",\"doi\":\"10.1002/ps.8634\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> BACKGROUND</h3>\\n \\n <p>The function of some testis-specific genes (TSGs) in model insects have been studied, but their function in non-model insects remains largely unexplored. In the present study, we identified several TSGs in the fall armyworm (FAW), a significant agricultural pest, through comparative transcriptomic analysis. A testis-specific gene cluster (TSGC) comprising multiple functional genes and long non-coding RNAs was found.</p>\\n </section>\\n \\n <section>\\n \\n <h3> RESULTS</h3>\\n \\n <p>Quantitative PCR analysis validated the testis-specific expression of genes within this cluster across different developmental stages. Interspecies collinearity analysis indicated that this TSGC is conserved among lepidopterans. To elucidate the function of this TSGC, we employed the CRISPR/Cas9 gene-editing technique to generate loss-of-function mutants. Notably, the hatching rate of eggs produced by wild-type females mated with mutant males (approximately 30%) was substantially lower than that of eggs from wild-type females mated with wild-type males (approximately 80%). Additionally, a significant reduction in the size and number of sperm was observed in the testis of mutant males compared to wild-type males.</p>\\n </section>\\n \\n <section>\\n \\n <h3> CONCLUSION</h3>\\n \\n <p>Collectively, this study not only elucidates the role of the TSGC in supporting male fertility in the FAW, which provides valuable information for understanding the mechanism of male reproduction in insects, but also identifies potential target genes for the development of genetic control strategies against lepidopteran pests. © 2025 Society of Chemical Industry.</p>\\n </section>\\n </div>\",\"PeriodicalId\":218,\"journal\":{\"name\":\"Pest Management Science\",\"volume\":\"81 4\",\"pages\":\"2355-2363\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-01-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pest Management Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/ps.8634\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pest Management Science","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/ps.8634","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

摘要

一些睾丸特异性基因（TSGs）在模式昆虫中的功能已经被研究过，但它们在非模式昆虫中的功能仍未被探索。在本研究中，我们通过比较转录组学分析鉴定了秋粘虫（FAW）的几个TSGs。发现了一个由多个功能基因和长链非编码rna组成的睾丸特异性基因簇（TSGC）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Knockout of a testis-specific gene cluster impairs male fertility in the fall armyworm, Spodoptera frugiperda

查看原文本刊更多论文

Knockout of a testis-specific gene cluster impairs male fertility in the fall armyworm, Spodoptera frugiperda

BACKGROUND

The function of some testis-specific genes (TSGs) in model insects have been studied, but their function in non-model insects remains largely unexplored. In the present study, we identified several TSGs in the fall armyworm (FAW), a significant agricultural pest, through comparative transcriptomic analysis. A testis-specific gene cluster (TSGC) comprising multiple functional genes and long non-coding RNAs was found.

RESULTS

Quantitative PCR analysis validated the testis-specific expression of genes within this cluster across different developmental stages. Interspecies collinearity analysis indicated that this TSGC is conserved among lepidopterans. To elucidate the function of this TSGC, we employed the CRISPR/Cas9 gene-editing technique to generate loss-of-function mutants. Notably, the hatching rate of eggs produced by wild-type females mated with mutant males (approximately 30%) was substantially lower than that of eggs from wild-type females mated with wild-type males (approximately 80%). Additionally, a significant reduction in the size and number of sperm was observed in the testis of mutant males compared to wild-type males.

CONCLUSION

Collectively, this study not only elucidates the role of the TSGC in supporting male fertility in the FAW, which provides valuable information for understanding the mechanism of male reproduction in insects, but also identifies potential target genes for the development of genetic control strategies against lepidopteran pests. © 2025 Society of Chemical Industry.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.