利用RNA-seq技术探索哥伦比亚三角瘤对拟除虫菊酯的新型抗性机制。

IF 2.2 Q1 ENTOMOLOGY
Sara Zuluaga , Geysson Javier Fernandez , Ana María Mejía-Jaramillo , Carl Lowenberger , Omar Triana-Chavez
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引用次数: 0

摘要

拟除虫菊酯是最广泛使用的杀虫剂,用于控制携带具有医学和经济意义的病原体的昆虫媒介。在哥伦比亚,有关triatomine杀虫剂耐药性的研究有限。由于杀虫剂抗性的挑战越来越大,本工作的重点是确定哥伦比亚三角眼蝇种群对不同拟除虫菊酯类杀虫剂的抗性。为了确定抗性的可能原因,研究了三种可能的分子机制:1)杀虫剂靶点电压门控钠通道基因(vgsc)编码区发生突变;2)与代谢抗性相关的酶活性调控;3)利用RNA-seq分析mRNA谱的变化。结果表明,田间种群对高效氯氟氰菊酯和溴氰菊酯均有抗性。在亚致死剂量杀虫剂下存活的昆虫没有表现出vgsc基因的经典突变。通过转录组学分析,发现在高效氯氟氰菊酯的选择压力下,田间种群和实验室种群的基因调控存在差异。基因富集分析显示,与解毒酶和线粒体蛋白相关的转录本正调控,可能在杀虫剂抗性中起重要作用。这项全面调查对于深入了解抗性机制和制定管理这些关键病媒物种的策略至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exploring novel pyrethroid resistance mechanisms through RNA-seq in Triatoma dimidiata from Colombia
Pyrethroids are the most widely used insecticides for controlling insect vectors carrying medically and economically significant pathogens. In Colombia, studies on triatomine insecticide resistance are limited. Due to the increasing challenge of insecticide resistance, this work focuses on determining resistance to different pyrethroid insecticides in populations of Triatoma dimidiata from Colombia. To define the possible causes of resistance, three potential molecular mechanisms were explored: 1) mutations in the coding region of the voltage-gated sodium channel gene (vgsc), the insecticide target site; 2) modulation of enzymatic activity associated with metabolic resistance; and 3) changes in the mRNA profiles using RNA-seq. The results showed that the field population of T. dimidiata was resistant to lambda-cyhalothrin and deltamethrin insecticides. Insects surviving sublethal doses of insecticides did not exhibit the classical mutations in the vgsc gene. Transcriptomic profile analyses of T. dimidiata revealed differentially regulated genes in field and laboratory populations under selective pressure with lambda-cyhalothrin. Gene enrichment analysis showed the positive regulation of transcripts related to detoxifying enzymes and mitochondrial proteins, which could play a significant role in insecticide resistance. This comprehensive investigation is crucial for providing insights into resistance mechanisms and generating strategies to manage these critical vector species.
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来源期刊
Current Research in Insect Science
Current Research in Insect Science Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
3.20
自引率
0.00%
发文量
22
审稿时长
36 days
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