Nilza Alves de Oliveira Jacobucci, M M Pinc, Mariana Dalmagro, Joice Karina Otênio Ribeiro, Talita Anielle de Assunção, Elissandro Jair Klein, Edson Antônio da Silva, Paula Derksen Macruz, Ezilda Jacomassi, Odair Alberton, Jaqueline Hoscheid
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Extractive optimization of bioactive compounds in aerial parts of cuphea carthagenensis using Box-Behnken experimental design.
Cuphea carthagenensis is known for its potential antioxidant and anti-inflammatory properties, attributed to triterpenes, flavonoids, and tannins. This study aimed to optimise the extraction process for aerial parts of C. carthagenensis using vortex extraction. A Box-Behnken experimental design coupled with response surface methodology was employed to evaluate the effects of three independent variables: sample-to-solvent ratio, velocity, and temperature. The optimised extract underwent phytochemical screening and assessment of its antimicrobial capacity. The highest extraction yields were obtained through maceration (22.84%) and ultrasound-assisted extraction (21.95%). However, the extract by vortex extraction exhibited significantly higher phenolic content (250.17 µgEAG gext-1) and antioxidant capacity. The optimal extraction conditions were identified as a sample-to-solvent ratio of 1:35, 12,000 rpm, and a temperature of 60 °C. The optimised extract was characterised by a predominance of glycosylated flavonoids and demonstrated moderate antibacterial activity, particularly against Streptococcus pyogenes, Staphylococcus epidermidis, and Bacillus subtilis.
期刊介绍：
The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds.
The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal.
Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section.
All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.
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