Sicong Zheng, Yudan Piao, Seung-Nam Jung, Chan Oh, Mi Ae Lim, QuocKhanh Nguyen, Shan Shen, Se-Hee Park, Shengzhe Cui, Shuyu Piao, Young Il Kim, Ji Won Kim, Ho-Ryun Won, Jae Won Chang, Yujuan Shan, Lihua Liu, Bon Seok Koo
{"title":"通过非热等离子体激活介质治疗放射耐药头颈部鳞状细胞癌改变基因表达谱。","authors":"Sicong Zheng, Yudan Piao, Seung-Nam Jung, Chan Oh, Mi Ae Lim, QuocKhanh Nguyen, Shan Shen, Se-Hee Park, Shengzhe Cui, Shuyu Piao, Young Il Kim, Ji Won Kim, Ho-Ryun Won, Jae Won Chang, Yujuan Shan, Lihua Liu, Bon Seok Koo","doi":"10.21053/ceo.2024.00238","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>High recurrence rates in head and neck squamous cell carcinoma (HNSCC) significantly affect prognosis, especially in radioresistant HNSCC (RR-HNSCC). Nonthermal plasma (NTP) therapy can effectively suppress the progression of HNSCC; however, the therapeutic mechanism of NTP therapy for RR-HNSCC remains unclear. In this study, we investigated the regulatory role of NTP in the RR-HNSCC signaling pathway and identified its signature genes.</p><p><strong>Methods: </strong>After constructing two RR-HNSCC cell lines, we prepared cell lysates from cells treated or not treated with non-thermal plasma-activated media (NTPAM) and performed RNA sequencing to determine their mRNA expression profiles. Based on the RNA sequencing.</p><p><strong>Results: </strong>we identified differentially expressed genes, followed by bioinformatics analysis to identify candidate molecules potentially associated with NTPAM therapy for RR-HNSCC.</p><p><strong>Results: </strong>NTPAM decreased RR-HNSCC cell viability in vitro. The RNA sequencing results indicated that NTPAM treatment activated the reactive oxygen species pathway and induced ferroptosis in RR-HNSCC cell lines. Among the 1924 genes correlated with radiation treatment, eight showed statistical significance in both the cell lines and The Cancer Genome Atlas (TCGA) cohort. Only five genes, ABCC3, DUSP16, PDGFB, RAF1, and THBS1, showed consistent results between the NTPAM data sequencing and TCGA data. LASSO regression analysis revealed that five genes were associated with cancer prognosis, with a hazard ratio (HR) of 2.26. In RR-HNSCC cells, NTPAM affected DUSP16, PDGFB, and THBS1 as the activated marker within 6 h and persisted for 12 h. Furthermore, enrichment analysis indicated that these three differential genes were associated with ECM, TGF-β, PI3K-AKT, and MET pathways.</p><p><strong>Conclusion: </strong>NTPAM therapy enhances cytotoxicity in RR-HNSCC cell lines by inducing specific ROS-mediated ferroptosis. DUSP16, PDGFB, and THBS1 were identified as crucial targets for reversing radiation resistance induced by NTPAM therapy, providing insights into the mechanisms and clinical applications of NTPAM treatment in RR-HNSCC.</p>","PeriodicalId":10318,"journal":{"name":"Clinical and Experimental Otorhinolaryngology","volume":" ","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Altered gene expression profiling by non-thermal plasma-activated media treatment in radioresistant head and neck squamous cell carcinoma.\",\"authors\":\"Sicong Zheng, Yudan Piao, Seung-Nam Jung, Chan Oh, Mi Ae Lim, QuocKhanh Nguyen, Shan Shen, Se-Hee Park, Shengzhe Cui, Shuyu Piao, Young Il Kim, Ji Won Kim, Ho-Ryun Won, Jae Won Chang, Yujuan Shan, Lihua Liu, Bon Seok Koo\",\"doi\":\"10.21053/ceo.2024.00238\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>High recurrence rates in head and neck squamous cell carcinoma (HNSCC) significantly affect prognosis, especially in radioresistant HNSCC (RR-HNSCC). Nonthermal plasma (NTP) therapy can effectively suppress the progression of HNSCC; however, the therapeutic mechanism of NTP therapy for RR-HNSCC remains unclear. In this study, we investigated the regulatory role of NTP in the RR-HNSCC signaling pathway and identified its signature genes.</p><p><strong>Methods: </strong>After constructing two RR-HNSCC cell lines, we prepared cell lysates from cells treated or not treated with non-thermal plasma-activated media (NTPAM) and performed RNA sequencing to determine their mRNA expression profiles. Based on the RNA sequencing.</p><p><strong>Results: </strong>we identified differentially expressed genes, followed by bioinformatics analysis to identify candidate molecules potentially associated with NTPAM therapy for RR-HNSCC.</p><p><strong>Results: </strong>NTPAM decreased RR-HNSCC cell viability in vitro. The RNA sequencing results indicated that NTPAM treatment activated the reactive oxygen species pathway and induced ferroptosis in RR-HNSCC cell lines. Among the 1924 genes correlated with radiation treatment, eight showed statistical significance in both the cell lines and The Cancer Genome Atlas (TCGA) cohort. Only five genes, ABCC3, DUSP16, PDGFB, RAF1, and THBS1, showed consistent results between the NTPAM data sequencing and TCGA data. LASSO regression analysis revealed that five genes were associated with cancer prognosis, with a hazard ratio (HR) of 2.26. In RR-HNSCC cells, NTPAM affected DUSP16, PDGFB, and THBS1 as the activated marker within 6 h and persisted for 12 h. Furthermore, enrichment analysis indicated that these three differential genes were associated with ECM, TGF-β, PI3K-AKT, and MET pathways.</p><p><strong>Conclusion: </strong>NTPAM therapy enhances cytotoxicity in RR-HNSCC cell lines by inducing specific ROS-mediated ferroptosis. DUSP16, PDGFB, and THBS1 were identified as crucial targets for reversing radiation resistance induced by NTPAM therapy, providing insights into the mechanisms and clinical applications of NTPAM treatment in RR-HNSCC.</p>\",\"PeriodicalId\":10318,\"journal\":{\"name\":\"Clinical and Experimental Otorhinolaryngology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-01-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical and Experimental Otorhinolaryngology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.21053/ceo.2024.00238\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"OTORHINOLARYNGOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical and Experimental Otorhinolaryngology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.21053/ceo.2024.00238","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OTORHINOLARYNGOLOGY","Score":null,"Total":0}
Altered gene expression profiling by non-thermal plasma-activated media treatment in radioresistant head and neck squamous cell carcinoma.
Objective: High recurrence rates in head and neck squamous cell carcinoma (HNSCC) significantly affect prognosis, especially in radioresistant HNSCC (RR-HNSCC). Nonthermal plasma (NTP) therapy can effectively suppress the progression of HNSCC; however, the therapeutic mechanism of NTP therapy for RR-HNSCC remains unclear. In this study, we investigated the regulatory role of NTP in the RR-HNSCC signaling pathway and identified its signature genes.
Methods: After constructing two RR-HNSCC cell lines, we prepared cell lysates from cells treated or not treated with non-thermal plasma-activated media (NTPAM) and performed RNA sequencing to determine their mRNA expression profiles. Based on the RNA sequencing.
Results: we identified differentially expressed genes, followed by bioinformatics analysis to identify candidate molecules potentially associated with NTPAM therapy for RR-HNSCC.
Results: NTPAM decreased RR-HNSCC cell viability in vitro. The RNA sequencing results indicated that NTPAM treatment activated the reactive oxygen species pathway and induced ferroptosis in RR-HNSCC cell lines. Among the 1924 genes correlated with radiation treatment, eight showed statistical significance in both the cell lines and The Cancer Genome Atlas (TCGA) cohort. Only five genes, ABCC3, DUSP16, PDGFB, RAF1, and THBS1, showed consistent results between the NTPAM data sequencing and TCGA data. LASSO regression analysis revealed that five genes were associated with cancer prognosis, with a hazard ratio (HR) of 2.26. In RR-HNSCC cells, NTPAM affected DUSP16, PDGFB, and THBS1 as the activated marker within 6 h and persisted for 12 h. Furthermore, enrichment analysis indicated that these three differential genes were associated with ECM, TGF-β, PI3K-AKT, and MET pathways.
Conclusion: NTPAM therapy enhances cytotoxicity in RR-HNSCC cell lines by inducing specific ROS-mediated ferroptosis. DUSP16, PDGFB, and THBS1 were identified as crucial targets for reversing radiation resistance induced by NTPAM therapy, providing insights into the mechanisms and clinical applications of NTPAM treatment in RR-HNSCC.
期刊介绍：
Clinical and Experimental Otorhinolaryngology (Clin Exp Otorhinolaryngol, CEO) is an international peer-reviewed journal on recent developments in diagnosis and treatment of otorhinolaryngology-head and neck surgery and dedicated to the advancement of patient care in ear, nose, throat, head, and neck disorders. This journal publishes original articles relating to both clinical and basic researches, reviews, and clinical trials, encompassing the whole topics of otorhinolaryngology-head and neck surgery.
CEO was first issued in 2008 and this journal is published in English four times (the last day of February, May, August, and November) per year by the Korean Society of Otorhinolaryngology-Head and Neck Surgery. The Journal aims at publishing evidence-based, scientifically written articles from different disciplines of otorhinolaryngology field.
The readership contains clinical/basic research into current practice in otorhinolaryngology, audiology, speech pathology, head and neck oncology, plastic and reconstructive surgery. The readers are otolaryngologists, head and neck surgeons and oncologists, audiologists, and speech pathologists.
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