人血吸虫病软体动物媒介中血吸虫抗性的基因组基础。

IF 4.6 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2025-01-17 DOI:10.1016/j.isci.2024.111520

Si-Ming Zhang , Guiyun Yan , Abdelmalek Lekired , Daibin Zhong

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引用次数: 0

摘要

淡水蜗牛是世界上最具破坏性的寄生虫病之一——血吸虫病传播的指定中间宿主。为了揭示蜗牛对血吸虫的抗性机制，我们从两个明确定义的纯合子系（iM系和iBS90）中培育出重组自交系（RILs）。采用全基因组测序（WGS）技术对46只RIL钉螺进行了基因组扫描，其中46只钉螺对曼氏血吸虫具有抗性或易感。在染色体水平组装基因组的辅助下，进行了全基因组关联研究（GWAS）和bin标记辅助数量性状位点（QTLs）分析。在5号染色体上发现了一个与血吸虫抗性相关的小基因组区域（约3mb），命名为B. glabrata schistosome resistance region 1 （BgSRR1）。本研究建立在我们最近开发的遗传和基因组资源的基础上，为研究抗血吸虫机制和未来发展以蜗牛为目标的血吸虫病生物防治计划提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Genomic basis of schistosome resistance in a molluscan vector of human schistosomiasis

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Genomic basis of schistosome resistance in a molluscan vector of human schistosomiasis

Freshwater snails are obligate intermediate hosts for the transmission of schistosomiasis, one of the world’s most devastating parasitic diseases. To decipher the mechanisms underlying snail resistance to schistosomes, recombinant inbred lines (RILs) were developed from two well-defined homozygous lines (iM line and iBS90) of the snail Biomphalaria glabrata. Whole-genome sequencing (WGS) was used to scan the genomes of 46 individual RIL snails, representing 46 RILs, half of which were resistant or susceptible to Schistosoma mansoni. Genome-wide association study (GWAS) and bin marker-assisted quantitative trait loci (QTLs) analysis, aided by our chromosome-level assembled genome, were conducted. A small genomic region (∼3 Mb) on chromosome 5 was identified as being associated with schistosome resistance, designated the B. glabrata schistosome resistance region 1 (BgSRR1). This study, built on our recently developed genetic and genomic resources, provides valuable insights into anti-schistosome mechanisms and the future development of snail-targeted biocontrol programs for schistosomiasis.

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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