{"title":"利用色谱-质谱联用代谢组学分析探讨环孢素治疗再生障碍性贫血的机制。","authors":"Ming-xin Guo, Lin Wan, Xiang Sun, Xi-han Zhou, Wen-tong Fang, Zhang-zhang Sun, Zhi-qiang Hu, Li-li Xue","doi":"10.1002/rcm.9968","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>The aim of this study was to use metabolomics techniques to detect differential metabolites in the plasma of patients with aplastic anemia (AA). We explore important biomarkers and potential pathways in cyclosporine A (CsA) in the treatment of AA.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Plasma samples from five patients with AA before and after treatment and plasma samples from five healthy people were collected and analyzed by liquid chromatography–mass spectrometry and gas chromatography–mass spectrometry. Multivariate statistical methods were employed to screen for differential compounds, followed by enrichment analysis of the differentially metabolites.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The experimental samples showed good stability and reproducibility. A total of 167 differential metabolites, including phospholipids, amino acids, and saturated or unsaturated fatty acids, were identified between AA patients and healthy individuals. Enrichment analysis of differential metabolites revealed the involvement of pathways such as pyrimidine metabolism, galactose metabolism, pantothenate and CoA biosynthesis, and forkhead box transcription factors signaling. A total of 26 differential metabolites were identified between AA patients and stable patients after treatment. Enrichment analysis of these metabolites showed the involvement of pathways such as pyrimidine metabolism, linoleic acid/<i>α</i>-linoleic acid metabolism, pantothenate and CoA biosynthesis, and beta-alanine metabolism.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Significant differences in metabolites were observed between AA patients and healthy individuals, suggesting that immune-related and energy metabolism pathways may be key targets in AA treatment. CsA intervention in AA may be achieved through the regulation of immune-related metabolic pathways.</p>\n </section>\n </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 6","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Metabolomics Analysis Using Chromatography–Mass Spectrometry to Investigate the Mechanism of Cyclosporine in the Treatment of Aplastic Anemia\",\"authors\":\"Ming-xin Guo, Lin Wan, Xiang Sun, Xi-han Zhou, Wen-tong Fang, Zhang-zhang Sun, Zhi-qiang Hu, Li-li Xue\",\"doi\":\"10.1002/rcm.9968\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>The aim of this study was to use metabolomics techniques to detect differential metabolites in the plasma of patients with aplastic anemia (AA). We explore important biomarkers and potential pathways in cyclosporine A (CsA) in the treatment of AA.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Plasma samples from five patients with AA before and after treatment and plasma samples from five healthy people were collected and analyzed by liquid chromatography–mass spectrometry and gas chromatography–mass spectrometry. Multivariate statistical methods were employed to screen for differential compounds, followed by enrichment analysis of the differentially metabolites.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The experimental samples showed good stability and reproducibility. A total of 167 differential metabolites, including phospholipids, amino acids, and saturated or unsaturated fatty acids, were identified between AA patients and healthy individuals. Enrichment analysis of differential metabolites revealed the involvement of pathways such as pyrimidine metabolism, galactose metabolism, pantothenate and CoA biosynthesis, and forkhead box transcription factors signaling. A total of 26 differential metabolites were identified between AA patients and stable patients after treatment. Enrichment analysis of these metabolites showed the involvement of pathways such as pyrimidine metabolism, linoleic acid/<i>α</i>-linoleic acid metabolism, pantothenate and CoA biosynthesis, and beta-alanine metabolism.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>Significant differences in metabolites were observed between AA patients and healthy individuals, suggesting that immune-related and energy metabolism pathways may be key targets in AA treatment. CsA intervention in AA may be achieved through the regulation of immune-related metabolic pathways.</p>\\n </section>\\n </div>\",\"PeriodicalId\":225,\"journal\":{\"name\":\"Rapid Communications in Mass Spectrometry\",\"volume\":\"39 6\",\"pages\":\"\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2025-01-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rapid Communications in Mass Spectrometry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/rcm.9968\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rapid Communications in Mass Spectrometry","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/rcm.9968","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Metabolomics Analysis Using Chromatography–Mass Spectrometry to Investigate the Mechanism of Cyclosporine in the Treatment of Aplastic Anemia
Objective
The aim of this study was to use metabolomics techniques to detect differential metabolites in the plasma of patients with aplastic anemia (AA). We explore important biomarkers and potential pathways in cyclosporine A (CsA) in the treatment of AA.
Methods
Plasma samples from five patients with AA before and after treatment and plasma samples from five healthy people were collected and analyzed by liquid chromatography–mass spectrometry and gas chromatography–mass spectrometry. Multivariate statistical methods were employed to screen for differential compounds, followed by enrichment analysis of the differentially metabolites.
Results
The experimental samples showed good stability and reproducibility. A total of 167 differential metabolites, including phospholipids, amino acids, and saturated or unsaturated fatty acids, were identified between AA patients and healthy individuals. Enrichment analysis of differential metabolites revealed the involvement of pathways such as pyrimidine metabolism, galactose metabolism, pantothenate and CoA biosynthesis, and forkhead box transcription factors signaling. A total of 26 differential metabolites were identified between AA patients and stable patients after treatment. Enrichment analysis of these metabolites showed the involvement of pathways such as pyrimidine metabolism, linoleic acid/α-linoleic acid metabolism, pantothenate and CoA biosynthesis, and beta-alanine metabolism.
Conclusion
Significant differences in metabolites were observed between AA patients and healthy individuals, suggesting that immune-related and energy metabolism pathways may be key targets in AA treatment. CsA intervention in AA may be achieved through the regulation of immune-related metabolic pathways.
期刊介绍：
Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.
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