结晶度降低的全甲壳素复合膜上癌细胞的粘附特性。

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2024-12-30 DOI:10.1016/j.carres.2024.109373

Masayasu Totani, Hiroyuki Shinchi, Jun-ichi Kadokawa

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引用次数: 0

摘要

我们先前制备了自增强几丁质复合材料（SR-ChC）薄膜，也称为全几丁质复合材料，由两种成分组成，即具有高结晶度的缩微几丁质纳米纤维（sd - chnf）和缩微低结晶度的几丁质（SD-LC-Ch）基质。在这项研究中，我们精确地评估了水环境条件下的亲水性及其对细胞粘附的影响，使用人源癌细胞在SR-ChC膜表面。对结晶度降低的SR-ChC膜进行表面分析，发现水中SD-LC-Ch组分的氨基分子链组装发生了重定向。因此，SD-LC-Ch组分的用量增加了膜表面亲水性氨基的数量，从而提高了细胞的粘附效率和延伸率。本研究得出结论，低晶SR-ChC膜中的亲水SD-LC-Ch成分有助于展示与生物底物（如细胞）相互作用有关的新功能。©2017 elsevierinc .版权所有

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Cancer cell adhesion property on all-chitin composite films with reduced crystallinity

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Cancer cell adhesion property on all-chitin composite films with reduced crystallinity

We previously prepared self-reinforced chitin composite (SR-ChC) films, also called all-chitin composites, comprising two components, that is, scale-down chitin nanofibers (SD-ChNFs) with high crystallinity and scale-down low-crystalline chitin (SD-LC-Ch) matrixes. In this study, we precisely evaluated hydrophilicity under water enviromental conditions and its effect on cell adhesion using human-derived cancer cells on the SR-ChC film surfaces. The surface analysis of the SR-ChC films with reduced crystallinity revealed reorientation of the molecular chain assemblies with amino groups in the SD-LC-Ch components in water. Consequently, the amount of the SD-LC-Ch components gave rise to increasing the number of hydrophilic amino groups on the film surfaces, which resulted in efficiency of cell adhesion and elongation. This study concludes that the hydrophilic SD-LC-Ch components in the lower crystalline SR-ChC films strongly contribute to exhibiting new functions, related to interaction with biological substrates, such as cells.

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".