凝结芽孢杆菌通过调节TLR4/MyD88/NF-κB信号通路减轻肺炎克雷伯菌诱导的家兔肠屏障损伤

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology Pub Date : 2025-02-01 DOI:10.1016/j.vetmic.2024.110364

Jianing Wang, Ziqiang Zhang, Jiajia Wang, Lihui Shi, Shuaishuai Wang, Bingyu Niu, Xiaonuo Tian, Qiongxia Lv, Lan Wei, Mengyun Li, Yumei Liu

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引用次数: 0

摘要

益生菌可有效缓解宿主腹泻，但具体机制尚不清楚。因此，我们通过HE、免疫荧光和16S rRNA等方法探讨了凝固芽孢杆菌（Bacillus coagulans， BC）对肺炎克雷伯菌（Klebsiella pneumoniae）致家兔肠道屏障损伤的保护机制。结果表明，BC预处理可缓解肺炎克雷伯菌对家兔平均日增重、平均日采食量和饲料代谢率的影响。此外，BC还能减轻肺炎克雷伯菌引起的家兔炎症细胞浸润、肠绒毛减少、隐窝加深和杯状细胞减少。进一步研究发现，BC通过改善肠道机械屏障、化学屏障、免疫屏障和微生物屏障来改善肠道屏障。具体来说，BC通过改善肠道结构、增加PCNA蛋白表达、增加杯状细胞数量、改变occludin、claudin-1和ZO-1的表达来改善肠道机械屏障。BC通过调节MUC1和MUC2的表达改善肠道化学屏障，通过改变炎症因子IL-1β、IL-6和TNF-α的表达水平抑制TLR4/MyD88/NF-κB信号通路，从而优化肠道免疫屏障。此外，饲粮中添加BC可通过降低有害菌的丰度和增加有益菌的丰度来改善家兔肠道微生物屏障。综上所述，BC通过改善肠道形态、减轻炎症反应和调节微生物组成来预防肺炎克雷伯菌诱导的肠道屏障损伤。其中，1 × 106 CFU/g的预处理效果最好。本研究为利用BC预防和治疗家兔肺炎克雷伯菌腹泻提供了理论依据。

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Bacillus coagulans alleviates intestinal barrier injury induced by Klebsiella pneumoniae in rabbits by regulating the TLR4/MyD88/NF-κB signalling pathway

Probiotics effectively alleviate host diarrhoea, but the specific mechanism is not clear. Therefore, we explored the protective mechanism of Bacillus coagulans (BC) on intestinal barrier injury induced by Klebsiella pneumoniae (K. pneumoniae) in rabbits by HE, immunofluorescence and 16S rRNA. The results showed that BC pretreatment alleviated the changes in average daily gain, average daily feed intake and FCR caused by K. pneumoniae in rabbits. Moreover, BC alleviated the inflammatory cell infiltration, intestinal villus reduction, crypt deepening and goblet cell reduction caused by K. pneumoniae in rabbits. Further research revealed that BC improved the intestinal barrier by improving the mechanical barrier, chemical barrier, immune barrier and microbial barrier. Specifically, BC improved the intestinal mechanical barrier by improving the intestinal structure, increasing the protein expression of PCNA, increasing the number of goblet cells, and altering the expression of occludin, claudin-1 and ZO-1. BC improved the intestinal chemical barrier by regulating the expression of MUC1 and MUC2 and inhibited the TLR4/MyD88/NF-κB signalling pathway by altering the expression levels of the inflammatory factors IL-1β, IL-6 and TNF-α, thus optimizing the intestinal immune barrier. In addition, adding BC to the diet improved the intestinal microbial barrier of rabbits by reducing the abundance of harmful bacteria and increasing the abundance of beneficial bacteria. In summary, BC protects against K. pneumoniae-induced intestinal barrier damage by improving intestinal morphology, mitigating the inflammatory response and regulating the microbial composition. Among the pretreatments, the pretreatment effect of 1 × 10⁶ CFU/g was the best. This study provides a theoretical basis for the use of BC to prevent and treat diarrhoea caused by K. pneumoniae in rabbits.

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.