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IF 3.7 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online Pub Date : 2024-10-15 DOI:10.1016/j.rbmo.2024.104480

Aşina Bayram, Neelke De Munck, Ibrahim Elkhatib, Erkan Kalafat, Andrea Abdala, Virginia Ferracuti, Laura Melado, Barbara Lawrenz, Human Fatemi, Daniela Nogueira

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引用次数: 0

摘要

研究问题：传统试管婴儿配子短期同卵培养是否会影响授精后的结果和胚胎形态动力学？2020年12月至2023年3月期间进行的同胞卵母细胞随机试验研究。符合条件的夫妇（n = 55）均为女性，年龄在 18-43 岁之间，体重指数在 35 千米/平方米或以下，男性精液参数正常。积层卵母细胞复合体（COC）（6 至 12 个）分别按 1:1:2 的比例随机分配到长（16-18 小时）或短（2 小时）共同孵育体外受精和卵胞浆内单精子显微注射中。所有通过体外受精授精的卵母细胞都接受了延时监测。在第5、6或7天对BL3CC或更高分级的囊胚进行滋养层活检，并通过新一代测序进行分析：共有954个COC被分配到长联合培养（n = 235 [24.6%]）、短联合培养（n = 235 [24.6%]）和ICSI（n = 484 [50.7%]）。其中，202 例经长联合培养后为 MII，209 例经短联合培养后为 MII，394 例经 ICSI 后为 MII。回归分析表明，与长时间联合培养相比，短时间联合培养组（OR 0.72，95% CI 0.46 至 1.11，P = 0.139）和 ICSI（OR 1.43，95% CI 0.95 至 2.15，P = 0.090）对正常受精率（2PN）没有显著影响。各组间每 MII、每 2PN 可用囊胚数，以及每 MII、每 2PN 和每检测囊胚的非整倍体囊胚数相似。胚胎停育率（危险比 [HR] 0.79，95% CI 0.57 至 1.11，P = 0.183）和胚胎达到形态发生阶段的时间在 IVF 组间相似（P > 0.05）：结论：与标准的隔夜共孵育相比，配子在试管婴儿中短时间共孵育 2 小时可获得相当的受精率、囊胚率和整倍体率，且不会对胚胎形态发生不利影响。

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Comparative analysis of long versus short co-incubation of gametes on post-insemination outcomes and embryo morphokinetics.

Research question: Does a short co-incubation of gametes in conventional IVF affect post-insemination outcomes and embryo morphokinetics?

Design: Sibling oocyte randomized pilot study conducted between December 2020 and March 2023. Eligible couples (n = 55) were women aged 18-43 years with BMI 35 km/m² or lower and male normal semen parameters. Cumulus oocyte complexes (COC) (six to 12) were randomized in a 1:1:2 proportion in long (16-18 h) or short (2 h) co-incubation IVF exposure and ICSI, respectively. All oocytes inseminated via IVF were monitored by time-lapse. Blastocysts graded BL3CC or higher underwent trophectoderm biopsy on days 5, 6 or 7, and were analysed by next-generation sequencing.

Results: A total of 954 COC were distributed to long co-incubation (n = 235 [24.6%]), short co-incubation (n = 235 [24.6%]) and ICSI (n = 484 [50.7%]). In total, 202 were MII after long co-incubation, 209 after short co-incubation and 394 at ICSI. Regression analyses showed that short co-incubation groups (OR 0.72, 95% CI 0.46 to 1.11, P = 0.139) and ICSI (OR 1.43, 95% CI 0.95 to 2.15, P = 0.090) did not significantly affect normal fertilization rates (2PN) compared with long co-incubation. Usable blastocysts per MII, per 2PN, and euploid blastocysts per MII, per 2PN and per tested blastocysts were similar among the groups. The rate of embryo arrest (hazard ratio [HR] 0.79, 95% CI 0.57 to 1.11, P = 0.183) and timings to reach morphokinetic milestones among embryos reaching blastulation were similar between IVF groups (P > 0.05 for all).

Conclusion: A short 2-h co-incubation of gametes in IVF yields comparable fertilization, blastocyst and euploidy rates without adverse embryo morphokinetic events, compared with standard overnight co-incubation.

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来源期刊

Reproductive biomedicine online 医学-妇产科学

CiteScore

7.20

自引率

7.50%

发文量

391

审稿时长

50 days

期刊介绍： Reproductive BioMedicine Online covers the formation, growth and differentiation of the human embryo. It is intended to bring to public attention new research on biological and clinical research on human reproduction and the human embryo including relevant studies on animals. It is published by a group of scientists and clinicians working in these fields of study. Its audience comprises researchers, clinicians, practitioners, academics and patients. Context: The period of human embryonic growth covered is between the formation of the primordial germ cells in the fetus until mid-pregnancy. High quality research on lower animals is included if it helps to clarify the human situation. Studies progressing to birth and later are published if they have a direct bearing on events in the earlier stages of pregnancy.