Zahra Ramezani, Mohammad Bagher Farhangi, Nasrin Ghorbanzadeh, Mahmoud Shabanpour
{"title":"大肠杆菌在两种酸性土壤中的迁移：微生物诱导方解石沉淀技术的影响。","authors":"Zahra Ramezani, Mohammad Bagher Farhangi, Nasrin Ghorbanzadeh, Mahmoud Shabanpour","doi":"10.1016/j.jconhyd.2024.104493","DOIUrl":null,"url":null,"abstract":"<p><p>Microbially induced calcite precipitation (MICP) while neutralizing soil pH, can lead to pore clogging which in turn may reduce bacteria transport. This study aimed to evaluate the effectiveness of the MICP process for E. coli filtration in two acidic soils. Two soil samples from Amlash (Am) and Lahijan (La) areas with pH values of 5.88 and 3.93, repectively, were collected and poured into plastic columns (14.92 × 2.4 cm). For the MICP reaction, the soil columns were saturated with a solidification solution (1:1 urea: CaCl<sub>2</sub>, 1.5 M) and Sporosarcina pasteurii (∼10<sup>8</sup> cell mL<sup>-1</sup>), and incubated at 30 °C for 72 h. Leaching experiments were conducted on both MICP-treated and control soil columns at steady-state, saturated flow condition. A pulse of influent (0.1 PV) containing Escherichia coli (ciprofloxacin-resistant) (∼10<sup>8</sup> cell mL<sup>-1</sup>) and bromide tracer (1000 mg L<sup>-1</sup> KBr) was added at the top of the soil columns, followed by sterile water to collect the effluent. Recovered E. coli, and Br<sup>-</sup>, HCO<sub>3</sub><sup>-</sup>, NH<sub>4</sub><sup>+</sup>, Ca<sup>2+</sup> ions were measured in the leachate. The profile of residual E. coli count, urease activity, and bioprecipitated CaCO<sub>3</sub> content were also assessed in the soil. Correlated with bioprecipitated CaCO<sub>3</sub>, the hydraulic conductivity coefficients (K<sub>s</sub>) was reduced by 4.4 and 5.8 times after MICP treatment in Am and La soils, respectively, thus bacteria leaching was significantly reduced. A higher filtration coefficient (λ<sub>f</sub>) and recovery rate of E. coli were calculated in the La soil column, likely due to the lower pH and higher anion exchange capacity, which induced greater bacterial mortality and electrostatic attraction, respectively. MICP treatment reduced the average and cumulative count of E. coli by ∼3.4 times compared to the control column. In conclusion, the application of MICP in acidic soil increased soil pH and reduced the risk of E. coli transport to deeper layers by reducing soil hydraulic conductivity.</p>","PeriodicalId":15530,"journal":{"name":"Journal of contaminant hydrology","volume":"269 ","pages":"104493"},"PeriodicalIF":3.5000,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Escherichia coli transport in two acidic soils: Effect of microbially induced calcite precipitation technology.\",\"authors\":\"Zahra Ramezani, Mohammad Bagher Farhangi, Nasrin Ghorbanzadeh, Mahmoud Shabanpour\",\"doi\":\"10.1016/j.jconhyd.2024.104493\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Microbially induced calcite precipitation (MICP) while neutralizing soil pH, can lead to pore clogging which in turn may reduce bacteria transport. This study aimed to evaluate the effectiveness of the MICP process for E. coli filtration in two acidic soils. Two soil samples from Amlash (Am) and Lahijan (La) areas with pH values of 5.88 and 3.93, repectively, were collected and poured into plastic columns (14.92 × 2.4 cm). For the MICP reaction, the soil columns were saturated with a solidification solution (1:1 urea: CaCl<sub>2</sub>, 1.5 M) and Sporosarcina pasteurii (∼10<sup>8</sup> cell mL<sup>-1</sup>), and incubated at 30 °C for 72 h. Leaching experiments were conducted on both MICP-treated and control soil columns at steady-state, saturated flow condition. A pulse of influent (0.1 PV) containing Escherichia coli (ciprofloxacin-resistant) (∼10<sup>8</sup> cell mL<sup>-1</sup>) and bromide tracer (1000 mg L<sup>-1</sup> KBr) was added at the top of the soil columns, followed by sterile water to collect the effluent. Recovered E. coli, and Br<sup>-</sup>, HCO<sub>3</sub><sup>-</sup>, NH<sub>4</sub><sup>+</sup>, Ca<sup>2+</sup> ions were measured in the leachate. The profile of residual E. coli count, urease activity, and bioprecipitated CaCO<sub>3</sub> content were also assessed in the soil. Correlated with bioprecipitated CaCO<sub>3</sub>, the hydraulic conductivity coefficients (K<sub>s</sub>) was reduced by 4.4 and 5.8 times after MICP treatment in Am and La soils, respectively, thus bacteria leaching was significantly reduced. A higher filtration coefficient (λ<sub>f</sub>) and recovery rate of E. coli were calculated in the La soil column, likely due to the lower pH and higher anion exchange capacity, which induced greater bacterial mortality and electrostatic attraction, respectively. MICP treatment reduced the average and cumulative count of E. coli by ∼3.4 times compared to the control column. In conclusion, the application of MICP in acidic soil increased soil pH and reduced the risk of E. coli transport to deeper layers by reducing soil hydraulic conductivity.</p>\",\"PeriodicalId\":15530,\"journal\":{\"name\":\"Journal of contaminant hydrology\",\"volume\":\"269 \",\"pages\":\"104493\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of contaminant hydrology\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jconhyd.2024.104493\",\"RegionNum\":3,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of contaminant hydrology","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.jconhyd.2024.104493","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Escherichia coli transport in two acidic soils: Effect of microbially induced calcite precipitation technology.
Microbially induced calcite precipitation (MICP) while neutralizing soil pH, can lead to pore clogging which in turn may reduce bacteria transport. This study aimed to evaluate the effectiveness of the MICP process for E. coli filtration in two acidic soils. Two soil samples from Amlash (Am) and Lahijan (La) areas with pH values of 5.88 and 3.93, repectively, were collected and poured into plastic columns (14.92 × 2.4 cm). For the MICP reaction, the soil columns were saturated with a solidification solution (1:1 urea: CaCl2, 1.5 M) and Sporosarcina pasteurii (∼108 cell mL-1), and incubated at 30 °C for 72 h. Leaching experiments were conducted on both MICP-treated and control soil columns at steady-state, saturated flow condition. A pulse of influent (0.1 PV) containing Escherichia coli (ciprofloxacin-resistant) (∼108 cell mL-1) and bromide tracer (1000 mg L-1 KBr) was added at the top of the soil columns, followed by sterile water to collect the effluent. Recovered E. coli, and Br-, HCO3-, NH4+, Ca2+ ions were measured in the leachate. The profile of residual E. coli count, urease activity, and bioprecipitated CaCO3 content were also assessed in the soil. Correlated with bioprecipitated CaCO3, the hydraulic conductivity coefficients (Ks) was reduced by 4.4 and 5.8 times after MICP treatment in Am and La soils, respectively, thus bacteria leaching was significantly reduced. A higher filtration coefficient (λf) and recovery rate of E. coli were calculated in the La soil column, likely due to the lower pH and higher anion exchange capacity, which induced greater bacterial mortality and electrostatic attraction, respectively. MICP treatment reduced the average and cumulative count of E. coli by ∼3.4 times compared to the control column. In conclusion, the application of MICP in acidic soil increased soil pH and reduced the risk of E. coli transport to deeper layers by reducing soil hydraulic conductivity.
期刊介绍：
The Journal of Contaminant Hydrology is an international journal publishing scientific articles pertaining to the contamination of subsurface water resources. Emphasis is placed on investigations of the physical, chemical, and biological processes influencing the behavior and fate of organic and inorganic contaminants in the unsaturated (vadose) and saturated (groundwater) zones, as well as at groundwater-surface water interfaces. The ecological impacts of contaminants transported both from and to aquifers are of interest. Articles on contamination of surface water only, without a link to groundwater, are out of the scope. Broad latitude is allowed in identifying contaminants of interest, and include legacy and emerging pollutants, nutrients, nanoparticles, pathogenic microorganisms (e.g., bacteria, viruses, protozoa), microplastics, and various constituents associated with energy production (e.g., methane, carbon dioxide, hydrogen sulfide).
The journal''s scope embraces a wide range of topics including: experimental investigations of contaminant sorption, diffusion, transformation, volatilization and transport in the surface and subsurface; characterization of soil and aquifer properties only as they influence contaminant behavior; development and testing of mathematical models of contaminant behaviour; innovative techniques for restoration of contaminated sites; development of new tools or techniques for monitoring the extent of soil and groundwater contamination; transformation of contaminants in the hyporheic zone; effects of contaminants traversing the hyporheic zone on surface water and groundwater ecosystems; subsurface carbon sequestration and/or turnover; and migration of fluids associated with energy production into groundwater.
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