Shasha Xu, Yi Zhai, Chen Wang, Yang Zhang, Xiaowei Liu, Jianjun Jiang, Yafei Mi
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However, there is a lack of relevant research on the relationship between plasma exosomes and AMI.</p><p><strong>Objective: </strong>This study was designed to explore the relationship between plasma exosomes and AMI.</p><p><strong>Methods: </strong>This retrospective study collected the basic clinical data of patients with AMI (n = 10), stable angina pectoris (SAP, n = 10), and noncoronary heart disease (CON, n = 10) at the Department of Cardiovascular Medicine at Taizhou Hospital (Zhejiang, China, 2021.01 to 2021.12). Proteomics was used to systematically screen the differential proteins of plasma exosomes in patients with clinical AMI, SAP, and CON. Then, the results were further verified using parallel reaction monitoring (PRM).</p><p><strong>Results: </strong>Five of all the differentially expressed proteins (DEPs) were quantified by PRM. Compared with the CON group, heparin cofactor 2 (SERPIND1), mannan-binding lectin serine protease 1 (MASP1), ficolin-2 (FCN2), and α1-Microglobulin/bikunin precursor (AMBP) were upregulated in patients with AMI and SAP, with a higher expression in AMI than in SAP. Additionally, human leukocyte antigen (HLA-C) was downregulated in both exosomes and plasma.</p><p><strong>Conclusion: </strong>The expression of four plasma exosome biomarkers in AMI and SAP patients was higher than that in noncoronary heart disease (NCHD) patients. HLA-C was downregulated in both exosomes and plasma, showing a potential to serve as a new candidate target for the detection and therapy of AMI.</p>","PeriodicalId":10984,"journal":{"name":"Current medicinal chemistry","volume":" ","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Proteomics Analysis of Five Potential Plasma-derived Exosomal Biomarkers for Acute Myocardial Infarction.\",\"authors\":\"Shasha Xu, Yi Zhai, Chen Wang, Yang Zhang, Xiaowei Liu, Jianjun Jiang, Yafei Mi\",\"doi\":\"10.2174/0109298673346007241113072114\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aims: </strong>This study was to explore the relationship between plasma exosomes and Acute myocardial infarction (AMI).</p><p><strong>Background: </strong>Acute myocardial infarction (AMI) is one of the most common cardiovascular complications. 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引用次数: 0
摘要
目的：探讨血浆外泌体与急性心肌梗死（AMI）的关系。背景：急性心肌梗死（AMI）是最常见的心血管并发症之一。最近的研究表明外泌体在心血管疾病的发生和发展中起着至关重要的作用。然而，血浆外泌体与AMI之间的关系缺乏相关研究。目的：探讨血浆外泌体与AMI的关系。方法：回顾性分析20121.01 ~ 20121.12年在浙江省台州市医院心血管内科就诊的AMI （n = 10）、稳定期心绞痛（SAP, n = 10）、非冠心病（CON, n = 10）患者的基本临床资料。采用蛋白质组学方法系统筛选临床AMI、SAP和con患者血浆外泌体的差异蛋白，并采用平行反应监测（PRM）进一步验证结果。结果：5个差异表达蛋白（DEPs）均通过PRM定量。与CON组相比，AMI和SAP患者的肝素辅助因子2 （SERPIND1）、甘露聚糖结合凝集素丝氨酸蛋白酶1 （MASP1）、ficolin-2 （FCN2）和α - 1微球蛋白/比库尼前体（AMBP）表达上调，AMI中的表达高于SAP。此外，外泌体和血浆中的人白细胞抗原（HLA-C）表达下调。结论：AMI和SAP患者血浆外泌体4种生物标志物的表达均高于非冠心病患者。HLA-C在外泌体和血浆中均下调，显示出作为AMI检测和治疗的新候选靶点的潜力。
Proteomics Analysis of Five Potential Plasma-derived Exosomal Biomarkers for Acute Myocardial Infarction.
Aims: This study was to explore the relationship between plasma exosomes and Acute myocardial infarction (AMI).
Background: Acute myocardial infarction (AMI) is one of the most common cardiovascular complications. Recent studies have shown that exosomes play a crucial role in the development and progression of cardiovascular diseases. However, there is a lack of relevant research on the relationship between plasma exosomes and AMI.
Objective: This study was designed to explore the relationship between plasma exosomes and AMI.
Methods: This retrospective study collected the basic clinical data of patients with AMI (n = 10), stable angina pectoris (SAP, n = 10), and noncoronary heart disease (CON, n = 10) at the Department of Cardiovascular Medicine at Taizhou Hospital (Zhejiang, China, 2021.01 to 2021.12). Proteomics was used to systematically screen the differential proteins of plasma exosomes in patients with clinical AMI, SAP, and CON. Then, the results were further verified using parallel reaction monitoring (PRM).
Results: Five of all the differentially expressed proteins (DEPs) were quantified by PRM. Compared with the CON group, heparin cofactor 2 (SERPIND1), mannan-binding lectin serine protease 1 (MASP1), ficolin-2 (FCN2), and α1-Microglobulin/bikunin precursor (AMBP) were upregulated in patients with AMI and SAP, with a higher expression in AMI than in SAP. Additionally, human leukocyte antigen (HLA-C) was downregulated in both exosomes and plasma.
Conclusion: The expression of four plasma exosome biomarkers in AMI and SAP patients was higher than that in noncoronary heart disease (NCHD) patients. HLA-C was downregulated in both exosomes and plasma, showing a potential to serve as a new candidate target for the detection and therapy of AMI.
期刊介绍：
Aims & Scope
Current Medicinal Chemistry covers all the latest and outstanding developments in medicinal chemistry and rational drug design. Each issue contains a series of timely in-depth reviews and guest edited thematic issues written by leaders in the field covering a range of the current topics in medicinal chemistry. The journal also publishes reviews on recent patents. Current Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments.
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