The geographic scale of population-level variation in growth and nodulation differs for two species of prairie clover

Premise

Prairies are among the most threatened biomes due to changing patterns of climate and land use, yet information on genetic variation in key species that would inform conservation is often limited. We assessed evidence for the geographic scale of population-level variation in growth of two species of prairie clover and of their symbiotic associations with nitrogen-fixing bacteria.

Methods

Seed representing two species, Dalea candida and D. purpurea, from the same five source populations were planted into an experimental site in Minnesota. We assessed variation within and among source populations in plant growth and in numbers of nodules and evaluated the relationship of growth and nodulation levels.

Results

Plant growth varied among source populations, with greater differences among populations of D. purpurea than of D. candida. We did not detect a relationship between plant growth and distance of source populations from the experimental site. Populations of both species were equally likely to develop nodules at the experimental site, but the numbers of nodules were lowest for the most distantly sourced populations. Plant growth was positively correlated with the number of nodules, and this relationship varied considerably within and among populations.

Conclusions

Environmental heterogeneity at local and regional scales maintains substantial levels of genetic variation in plant populations within remnant prairie preserves. Further, association with rhizobia at a restoration site can improve growth of widely sourced plant populations. The in situ maintenance of plant genetic variation and species diversity provides resources for conservation and maintenance of prairie biomes.