从特应性皮炎患儿粪便中分离的隐状副芽孢杆菌

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2025-01-05 DOI:10.1007/s00203-024-04221-y

Andrei V. Chaplin, Alla P. Pikina, Victoria A. Shcherbakova, Sofia R. Sokolova, Irina V. Podoprigora, Milana S. Das, Filipp A. Koshkin, Galit A. Ilyina, Iuliia A. Vasiliadis, Boris A. Efimov

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引用次数: 0

摘要

从一名患有特应性皮炎的3岁男童的粪便中分离出一株专性厌氧革兰氏阴性杆状细菌AD58T。最接近的种是粪副杆菌，其16S rRNA基因同源性为96.98%。AD58T与粪p.a fealis AGMB00274T的平均核苷酸同源值为85.0 ~ 85.4%。基因组长3.77 Mbp， G + C含量为43.5 mol%。菌株AD58T生长温度为32 ~ 42℃，pH值为6.0 ~ 7.5。在1%或更高的NaCl浓度下，没有观察到生长。主要脂肪酸为前iso-C15: 0、iso-C15: 0和总特征11 (iso-C17: 0 3-OH和/或C18: 2 DMA)，主要呼吸醌为MK-9。AD58T条件培养基提高了HT29细胞中IL-8的表达，降低了TLR-4的表达。基于上述特征，我们提出AD58T为潜隐副杆菌sp. 11 （= VKM B-3630T = JCM 35468T）的类型菌株。

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Parabacteroides absconsus sp. nov., isolated from feces of a child with atopic dermatitis

An obligate anaerobic, Gram-negative rod-shaped bacterial strain designated AD58^T was isolated from the feces of a 3-year-old boy with atopic dermatitis. The closest species is Parabacteroides fecalis with 96.98% 16S rRNA gene identity. The average nucleotide identity value between AD58^T and P. fecalis AGMB00274^T is 85.0–85.4%. The circular genome sequence is 3.77 Mbp long with 43.5 mol% G + C content. The strain AD58^T grows at 32–42 °C, its pH range for growth is 6.0–7.5. No growth is observed in the presence of 1% or higher NaCl concentrations. The major fatty acids are anteiso-C15: 0, iso-C15: 0, and summed feature 11 (iso-C17: 0 3–OH and/or C18: 2 DMA), and the predominant respiratory quinone is MK-9. Conditioned media from AD58^T increased expression of IL-8 but decreased expression of TLR-4 in HT29 cells. Based on the described properties, we propose AD58^T as the type strain of Parabacteroides absconsus sp. nov. (= VKM B-3630^T = JCM 35468^T).

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.