IF 3.8 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Karina Kizjakina, Yumin Dai, Pablo Sobrado
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引用次数: 0

摘要

我们报告了一种新的光依赖性 2-卤丙烯酸酯氢化酶(2HAH)的活化机制,这是一种黄素-脱氧内酯脱卤酶。最初的测定显示酶活性不稳定,只有在化学还原或暴露在强光下后才会稳定。光谱分析显示,光能加速 NAD(P)H 对黄素的还原,在强光下 30 秒就能完成还原,而在黑暗中还原速度较慢。蓝光能特异性地引发完全活化,而红光则没有影响。序列和结构分析表明,2HAH 与已知的光敏黄素蛋白不具有同源性,这表明存在一种未定性的调控机制。这些发现加深了我们对黄素酶调控的理解,并将光作为调节2HAH活性的一种潜在工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Light regulation of flavin reduction by NAD(P)H: activation of 2-haloacrylate hydratase.

We report a novel light-dependent activation mechanism for 2-haloacrylate hydratase (2HAH), a flavin-depu change endent dehalogenase. Initial assays revealed inconsistent enzyme activity, stabilized only after chemical reduction or exposure to bright light. Spectroscopic analysis showed that light accelerates flavin reduction by NAD(P)H, completing in 30 seconds under bright light versus slow reduction in the dark. Blue light specifically triggered full activation, while red light had no effect. Sequence and structural analyses indicate that 2HAH does not share homology with known light-sensitive flavoproteins, suggesting an uncharacterized regulatory mechanism. These findings advance our understanding of flavin enzyme regulation and introduce light as a potential tool for modulating 2HAH activity.

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来源期刊
Archives of biochemistry and biophysics
Archives of biochemistry and biophysics 生物-生化与分子生物学
CiteScore
7.40
自引率
0.00%
发文量
245
审稿时长
26 days
期刊介绍: Archives of Biochemistry and Biophysics publishes quality original articles and reviews in the developing areas of biochemistry and biophysics. Research Areas Include: • Enzyme and protein structure, function, regulation. Folding, turnover, and post-translational processing • Biological oxidations, free radical reactions, redox signaling, oxygenases, P450 reactions • Signal transduction, receptors, membrane transport, intracellular signals. Cellular and integrated metabolism.
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