{"title":"用对氧磷酶-1调节溶血磷脂：探讨其对炎症反应和免疫反应的影响。","authors":"Takuya Shimura, Hideaki Isago, Yoshifumi Morita, Ryunosuke Ohkawa, Naoyuki Yoshikawa, Yoshikazu Ono, Makoto Kurano","doi":"10.1016/j.bbrc.2024.151234","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Paraoxonase-1 (PON1) is a crucial esterase in cardiovascular health, closely associated with HDL and known for its antioxidant and anti-inflammatory properties. Reduced PON1 activity has been linked to cardiovascular diseases. Lysophospholipids (LysoPLs), essential for cellular processes and immune responses, are implicated in the pathogenesis of cardiovascular diseases and are bound to lipoproteins, contributing to their diverse effects. Thus, we hypothesize that the relationship between PON1 and cardiovascular diseases may involve the modulation of LysoPLs by PON1. This study aims to investigate how PON1 potentially influences LysoPLs.</p><p><strong>Methods: </strong>We quantified the levels of LysoPLs in HepG2 cells by using liquid chromatography-mass spectrometry, manipulating PON1 expression or knockdown.</p><p><strong>Results: </strong>In cells overexpressing PON1, there was a significant increase in cellular levels of lysophosphatidylserine (LysoPS) and medium levels of LysoPS. Conversely, in cells with PON-1 knockdown, cellular levels of lysophosphatidylcholine (LysoPC) and medium levels of LysoPC showed a significant decrease.</p><p><strong>Conclusions: </strong>PON1 is involved in modulating LysoPLs, which contribute to the antioxidant and anti-inflammatory properties of HDL, often attributed to PON1.</p>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"746 ","pages":"151234"},"PeriodicalIF":2.5000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Modulating lysophospholipids with Paraoxonase-1: Exploring its impact on inflammatory responses and immune reactions.\",\"authors\":\"Takuya Shimura, Hideaki Isago, Yoshifumi Morita, Ryunosuke Ohkawa, Naoyuki Yoshikawa, Yoshikazu Ono, Makoto Kurano\",\"doi\":\"10.1016/j.bbrc.2024.151234\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Paraoxonase-1 (PON1) is a crucial esterase in cardiovascular health, closely associated with HDL and known for its antioxidant and anti-inflammatory properties. Reduced PON1 activity has been linked to cardiovascular diseases. Lysophospholipids (LysoPLs), essential for cellular processes and immune responses, are implicated in the pathogenesis of cardiovascular diseases and are bound to lipoproteins, contributing to their diverse effects. Thus, we hypothesize that the relationship between PON1 and cardiovascular diseases may involve the modulation of LysoPLs by PON1. This study aims to investigate how PON1 potentially influences LysoPLs.</p><p><strong>Methods: </strong>We quantified the levels of LysoPLs in HepG2 cells by using liquid chromatography-mass spectrometry, manipulating PON1 expression or knockdown.</p><p><strong>Results: </strong>In cells overexpressing PON1, there was a significant increase in cellular levels of lysophosphatidylserine (LysoPS) and medium levels of LysoPS. Conversely, in cells with PON-1 knockdown, cellular levels of lysophosphatidylcholine (LysoPC) and medium levels of LysoPC showed a significant decrease.</p><p><strong>Conclusions: </strong>PON1 is involved in modulating LysoPLs, which contribute to the antioxidant and anti-inflammatory properties of HDL, often attributed to PON1.</p>\",\"PeriodicalId\":8779,\"journal\":{\"name\":\"Biochemical and biophysical research communications\",\"volume\":\"746 \",\"pages\":\"151234\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemical and biophysical research communications\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1016/j.bbrc.2024.151234\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/12/25 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical and biophysical research communications","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.bbrc.2024.151234","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/25 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Modulating lysophospholipids with Paraoxonase-1: Exploring its impact on inflammatory responses and immune reactions.
Introduction: Paraoxonase-1 (PON1) is a crucial esterase in cardiovascular health, closely associated with HDL and known for its antioxidant and anti-inflammatory properties. Reduced PON1 activity has been linked to cardiovascular diseases. Lysophospholipids (LysoPLs), essential for cellular processes and immune responses, are implicated in the pathogenesis of cardiovascular diseases and are bound to lipoproteins, contributing to their diverse effects. Thus, we hypothesize that the relationship between PON1 and cardiovascular diseases may involve the modulation of LysoPLs by PON1. This study aims to investigate how PON1 potentially influences LysoPLs.
Methods: We quantified the levels of LysoPLs in HepG2 cells by using liquid chromatography-mass spectrometry, manipulating PON1 expression or knockdown.
Results: In cells overexpressing PON1, there was a significant increase in cellular levels of lysophosphatidylserine (LysoPS) and medium levels of LysoPS. Conversely, in cells with PON-1 knockdown, cellular levels of lysophosphatidylcholine (LysoPC) and medium levels of LysoPC showed a significant decrease.
Conclusions: PON1 is involved in modulating LysoPLs, which contribute to the antioxidant and anti-inflammatory properties of HDL, often attributed to PON1.
期刊介绍：
Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination of timely and significant experimental results in diverse fields of biological research. The development of the "Breakthroughs and Views" section brings the minireview format to the journal, and issues often contain collections of special interest manuscripts. BBRC is published weekly (52 issues/year).Research Areas now include: Biochemistry; biophysics; cell biology; developmental biology; immunology
; molecular biology; neurobiology; plant biology and proteomics
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