后向散射穆勒矩阵偏振法估计复杂脑组织结构的微尺度各向异性和取向。

IF 1.9 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Journal of Medical Imaging Pub Date : 2025-01-01 Epub Date: 2024-12-31 DOI:10.1117/1.JMI.12.1.016001
Rhea Carlson, Courtney Comrie, Justina Bonaventura, Kellys Morara, Noelle Daigle, Elizabeth Hutchinson, Travis W Sawyer
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引用次数: 0

摘要

目的:弥散性磁共振成像(dMRI)定量评估脑微观结构,弥散性磁共振成像是临床应用的一种框架。为了推进这种dMRI方法,必须对微尺度各向异性和取向进行直接定量比较。完全后向散射穆勒矩阵偏振光成像(PLI)使薄和厚的组织标本的成像,以获得许多光学指标不可能通过传统的传输PLI方法。通过在雪貂视交叉(OC)内比较完整的PLI和dMRI,我们可以研究这种PLI技术作为dMRI验证工具的潜力,并深入了解这种成像方法在不同组织厚度下的显微结构和取向灵敏度。方法:采用dMRI和完全后向散射Mueller矩阵PLI对死后的雪貂脑组织(全脑1例,OC 3例)进行成像。对标本进行切片,然后用PLI重新成像。对相干视神经和交叉交叉的dMRI和PLI的标量度量和方向向量进行兴趣区和相关性分析。结果:光学延迟和dMRI分数各向异性在度量值之间表现出相似的趋势,并且强相关,表明延迟偏向宏观尺度结构。厚组织在双衰减角和dMRI纤维取向分布符号之间表现出相似的取向,而在延迟角中不明显。结论:我们证明了后向散射穆勒矩阵PLI显示了作为厚组织标本显微结构dMRI验证工具的潜力。进行完整的偏振测量可以提供方向表征和潜在的微尺度各向异性信息,而传统的PLI无法单独提供这些信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Backscattering Mueller matrix polarimetry estimates microscale anisotropy and orientation in complex brain tissue structure.

Purpose: Diffusion magnetic resonance imaging (dMRI) quantitatively estimates brain microstructure, diffusion tractography being one clinically utilized framework. To advance such dMRI approaches, direct quantitative comparisons between microscale anisotropy and orientation are imperative. Complete backscattering Mueller matrix polarized light imaging (PLI) enables the imaging of thin and thick tissue specimens to acquire numerous optical metrics not possible through conventional transmission PLI methods. By comparing complete PLI to dMRI within the ferret optic chiasm (OC), we may investigate the potential of this PLI technique as a dMRI validation tool and gain insight into the microstructural and orientational sensitivity of this imaging method in different tissue thicknesses.

Approach: Post-mortem ferret brain tissue samples (whole brain, n = 1 and OC, n = 3 ) were imaged with both dMRI and complete backscattering Mueller matrix PLI. The specimens were sectioned and then reimaged with PLI. Region of interest and correlation analyses were performed on scalar metrics and orientation vectors of both dMRI and PLI in the coherent optic nerve and crossing chiasm.

Results: Optical retardance and dMRI fractional anisotropy showed similar trends between metric values and were strongly correlated, indicating a bias to macroscale architecture in retardance. Thick tissue displays comparable orientation between the diattenuation angle and dMRI fiber orientation distribution glyphs that are not evident in the retardance angle.

Conclusions: We demonstrate that backscattering Mueller matrix PLI shows potential as a tool for microstructural dMRI validation in thick tissue specimens. Performing complete polarimetry can provide directional characterization and potentially microscale anisotropy information not available by conventional PLI alone.

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来源期刊
Journal of Medical Imaging
Journal of Medical Imaging RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
4.10
自引率
4.20%
发文量
0
期刊介绍: JMI covers fundamental and translational research, as well as applications, focused on medical imaging, which continue to yield physical and biomedical advancements in the early detection, diagnostics, and therapy of disease as well as in the understanding of normal. The scope of JMI includes: Imaging physics, Tomographic reconstruction algorithms (such as those in CT and MRI), Image processing and deep learning, Computer-aided diagnosis and quantitative image analysis, Visualization and modeling, Picture archiving and communications systems (PACS), Image perception and observer performance, Technology assessment, Ultrasonic imaging, Image-guided procedures, Digital pathology, Biomedical applications of biomedical imaging. JMI allows for the peer-reviewed communication and archiving of scientific developments, translational and clinical applications, reviews, and recommendations for the field.
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