{"title":"研究选定的B族维生素(B1、B2、B6和B12)对实验性大鼠急性结肠炎的影响。","authors":"Niloufar Dalayeli, Valiollah Hajhashemi, Ardeshir Talebi, Mohsen Minaiyan","doi":"10.4103/ijpvm.ijpvm_232_23","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Malnutrition and lack of micronutrients are seen in many patients with ulcerative colitis (UC). Considering that vitamins play an important role in preventing or protecting against inflammation at the metabolic, physiological, and pathological levels, this research was aimed to investigate the effect of a number of vitamin B groups in improving UC.</p><p><strong>Methods: </strong>Experimental colitis was induced by rectal administration of acetic acid (3%) in male Wistar rats, and mega doses of thiamine (20 mg/kg), riboflavin (15 mg/kg), pyridoxine (30 mg/kg), and cyanocobalamin (250 μg/kg) alone or in combination were administered intraperitoneally for 5 days. In another group, normal dose (1/10 of the above-mentioned doses) of four vitamins in combination was administered for 15 days (started 10 days before colitis induction). Colon tissues were weighted and evaluated in terms of macroscopic, microscopic, and biochemical markers.</p><p><strong>Results: </strong>Normal dose of four vitamins in combination form and mega dose of thiamine caused a significant increase in the body weight of animals. All treatments except for pyridoxine (mega dose) diminished ulceration index, total colitis index, and colon weight compared to the control group. Myeloperoxidase (MPO) activity and malondialdehyde (MDA) levels decreased significantly in all treatment groups.</p><p><strong>Conclusions: </strong>The anti-inflammatory and anti-ulcerative properties of selected B vitamins improved experimental colitis regardless of the dosage and duration of treatment. Despite its beneficial effect on biochemical markers, pyridoxine had the least effect in reducing the pathological features of colitis. More studies are needed to confirm the effect of these vitamins in the clinical setting of this disease.</p>","PeriodicalId":14342,"journal":{"name":"International Journal of Preventive Medicine","volume":"15 ","pages":"61"},"PeriodicalIF":1.7000,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687687/pdf/","citationCount":"0","resultStr":"{\"title\":\"Investigating the Impact of Selected B Vitamins (B1, B2, B6, and B12) on Acute Colitis Induced Experimentally in Rats.\",\"authors\":\"Niloufar Dalayeli, Valiollah Hajhashemi, Ardeshir Talebi, Mohsen Minaiyan\",\"doi\":\"10.4103/ijpvm.ijpvm_232_23\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Malnutrition and lack of micronutrients are seen in many patients with ulcerative colitis (UC). Considering that vitamins play an important role in preventing or protecting against inflammation at the metabolic, physiological, and pathological levels, this research was aimed to investigate the effect of a number of vitamin B groups in improving UC.</p><p><strong>Methods: </strong>Experimental colitis was induced by rectal administration of acetic acid (3%) in male Wistar rats, and mega doses of thiamine (20 mg/kg), riboflavin (15 mg/kg), pyridoxine (30 mg/kg), and cyanocobalamin (250 μg/kg) alone or in combination were administered intraperitoneally for 5 days. In another group, normal dose (1/10 of the above-mentioned doses) of four vitamins in combination was administered for 15 days (started 10 days before colitis induction). Colon tissues were weighted and evaluated in terms of macroscopic, microscopic, and biochemical markers.</p><p><strong>Results: </strong>Normal dose of four vitamins in combination form and mega dose of thiamine caused a significant increase in the body weight of animals. All treatments except for pyridoxine (mega dose) diminished ulceration index, total colitis index, and colon weight compared to the control group. Myeloperoxidase (MPO) activity and malondialdehyde (MDA) levels decreased significantly in all treatment groups.</p><p><strong>Conclusions: </strong>The anti-inflammatory and anti-ulcerative properties of selected B vitamins improved experimental colitis regardless of the dosage and duration of treatment. Despite its beneficial effect on biochemical markers, pyridoxine had the least effect in reducing the pathological features of colitis. More studies are needed to confirm the effect of these vitamins in the clinical setting of this disease.</p>\",\"PeriodicalId\":14342,\"journal\":{\"name\":\"International Journal of Preventive Medicine\",\"volume\":\"15 \",\"pages\":\"61\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-11-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687687/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Preventive Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/ijpvm.ijpvm_232_23\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICINE, GENERAL & INTERNAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Preventive Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/ijpvm.ijpvm_232_23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
Investigating the Impact of Selected B Vitamins (B1, B2, B6, and B12) on Acute Colitis Induced Experimentally in Rats.
Background: Malnutrition and lack of micronutrients are seen in many patients with ulcerative colitis (UC). Considering that vitamins play an important role in preventing or protecting against inflammation at the metabolic, physiological, and pathological levels, this research was aimed to investigate the effect of a number of vitamin B groups in improving UC.
Methods: Experimental colitis was induced by rectal administration of acetic acid (3%) in male Wistar rats, and mega doses of thiamine (20 mg/kg), riboflavin (15 mg/kg), pyridoxine (30 mg/kg), and cyanocobalamin (250 μg/kg) alone or in combination were administered intraperitoneally for 5 days. In another group, normal dose (1/10 of the above-mentioned doses) of four vitamins in combination was administered for 15 days (started 10 days before colitis induction). Colon tissues were weighted and evaluated in terms of macroscopic, microscopic, and biochemical markers.
Results: Normal dose of four vitamins in combination form and mega dose of thiamine caused a significant increase in the body weight of animals. All treatments except for pyridoxine (mega dose) diminished ulceration index, total colitis index, and colon weight compared to the control group. Myeloperoxidase (MPO) activity and malondialdehyde (MDA) levels decreased significantly in all treatment groups.
Conclusions: The anti-inflammatory and anti-ulcerative properties of selected B vitamins improved experimental colitis regardless of the dosage and duration of treatment. Despite its beneficial effect on biochemical markers, pyridoxine had the least effect in reducing the pathological features of colitis. More studies are needed to confirm the effect of these vitamins in the clinical setting of this disease.
期刊介绍:
International Journal of Preventive Medicine, a publication of Isfahan University of Medical Sciences, is a peer-reviewed online journal with Continuous print on demand compilation of issues published. The journal’s full text is available online at http://www.ijpvmjournal.net. The journal allows free access (Open Access) to its contents and permits authors to self-archive final accepted version of the articles on any OAI-compliant institutional / subject-based repository. The journal will cover technical and clinical studies related to health, ethical and social issues in field of Preventive Medicine. Articles with clinical interest and implications will be given preference.