饲料中添加石榴皮多酚对金鲳鱼抗氧化能力、免疫、脂质代谢和肝肠健康的影响

IF 2.4 3区 农林科学 Q2 FISHERIES
Guangye Liu, Zhoulin Yu, Sijie Li, Shuyan Zhao, Meng Zhou, Xiaohong Tan
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引用次数: 0

摘要

研究了石榴皮多酚(PPPs)对卵形沙眼生长性能、抗氧化能力、脂质代谢、免疫功能以及肝脏和肠道形态的影响。试验分为6组,每组在基础日粮中添加不同浓度的PPPs。剂量分别为0、10、30、90、270、810 mg/kg,分别命名为P0、P1、P2、P3、P4、P5。试验期56 d。试验共选取270尾鱼,初始体重为33.17±0.79 g,随机分配到18个浮动网箱中,每个网箱15尾鱼。结果显示,P3组和P4组肝体指数明显低于对照组(P < 0.05)。各实验组甘油三酯(TG)降低,高密度脂蛋白(HDL-C)升高,低密度脂蛋白(LDL-C)降低(P < 0.05)。此外,与对照组(P0)相比,PPPs增强了卵形沙眼的抗氧化能力,表现为血清超氧化物歧化酶(SOD)和总抗氧化能力(T-AOC)水平升高,丙二醛(MDA)含量降低。过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、HO-1、Nrf-2 mRNA表达量升高,而Keap-1表达量降低。在免疫方面,ppp对金鲳鱼有有益的作用。血清补体3 (C3)、补体4 (C4)、溶菌酶(LZM)水平升高,IL-10 mRNA表达增强,IL-8、NF-κB表达降低。在脂质代谢方面,与对照组相比,PPPs的添加导致肝脏脂质代谢基因Fas mRNA水平降低,CPT1、Apob-100和PPARα表达增加。最后,PPPs减轻了卵形沙眼肝脏空泡化,增加了卵形沙眼肠绒毛的高度和宽度。综上所述,加入PPPs可有效改善卵形沙眼肝脏脂肪积累、抗氧化能力和非特异性免疫。根据本研究结果,建议卵形沙眼饲料中PPPs的添加量为270 mg/kg。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effects of dietary pomegranate peel polyphenols on antioxidant capacity, immunity, lipid metabolism, and hepatointestinal health for golden pompano (Trachinotus ovatus)

Effects of dietary pomegranate peel polyphenols on antioxidant capacity, immunity, lipid metabolism, and hepatointestinal health for golden pompano (Trachinotus ovatus)

Effects of dietary pomegranate peel polyphenols on antioxidant capacity, immunity, lipid metabolism, and hepatointestinal health for golden pompano (Trachinotus ovatus)

The study investigated the effects of pomegranate peel polyphenols (PPPs) regarding growth performance, antioxidant capacity, lipid metabolism, immune function, and liver and intestine morphology in Trachinotus ovatus. The trial consisted of 6 groups, each receiving different concentrations of PPPs in their basal diet. The dosages used were 0, 10, 30, 90, 270, and 810 mg/kg, designated as P0, P1, P2, P3, P4, and P5, respectively. The feeding trial lasted for 56 days. In total, 270 fish, initially weighing 33.17 ± 0.79 g, were selected and randomly allocated to 18 floating cages, with 15 fish in each cage. The outcomes demonstrated that the hepatosomatic index of the P3 and P4 groups reduced considerably than the control group (P0) (P < 0.05). The triglyceride (TG) in each experimental group decreased, while high-density lipoprotein (HDL-C) increased in the P4 and P5 groups, and low-density lipoprotein (LDL-C) decreased (P < 0.05).Furthermore, PPPs were found to enhance the antioxidant capacity of Trachinotus ovatus than the control group (P0), manifesting as an increase in serum superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) levels and a decrease in malondialdehyde (MDA) content. Meanwhile, the mRNA expression levels of catalase (CAT), SOD, glutathione peroxidase (GSH-PX), HO-1, and Nrf-2 were increased, while the expression of Keap-1 was decreased. Regarding immunity, PPPs had a beneficial effect on the golden pompano. The levels of complement 3 (C3), complement 4 (C4), and lysozyme (LZM) in the serum were higher, while the IL-10 mRNA expression was enhanced, and the IL-8 and NF-κB expression levels were decreased compared to the P0 group. In relation to lipid metabolism, the addition of PPPs led to a reduction in the mRNA levels of the liver lipid metabolism gene Fas and an increase in the expression of CPT1, Apob-100, and PPARα, as compared to the control group. Lastly, PPPs mitigated liver vacuolization and enhanced the height and width of intestinal villi in Trachinotus ovatus. In summary, the incorporation of PPPs effectively improved liver fat accumulation, antioxidant capacity, and non-specific immunity in Trachinotus ovatus. Based on the results of this study, we recommend that the addition amount of PPPs in the feed of Trachinotus ovatus is 270 mg/kg.

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来源期刊
Aquaculture International
Aquaculture International 农林科学-渔业
CiteScore
5.10
自引率
6.90%
发文量
204
审稿时长
1.0 months
期刊介绍: Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.
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