Small molecules inhibiting EPHEMERAL1 to extend flower longevity by regulating petal senescence.

Key message: Everlastin1 and Everlastin2, potent inhibitors of EPH1, were identified through a wheat cell-free chemical-screening system. This innovative platform enables the development of small molecules that target 'undruggable' transcription factors. By specifically targeting the EPH1 pathway, these inhibitors delay petal senescence, extending the longevity and quality of ornamental flowers. This approach offers a precise alternative to traditional postharvest treatments. Moreover, this chemical discovery strategy can be applied to develop inhibitors for other agriculturally important traits and disease-related transcription factors, opening up broad applications in floriculture, agriculture, and beyond.