间质性膀胱炎/膀胱疼痛综合征的匹配血清和尿液生物标志物。

IF 2.6 3区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
PLoS ONE Pub Date : 2024-12-31 eCollection Date: 2024-01-01 DOI:10.1371/journal.pone.0309815
Tadeja Kuret, Igor Sterle, Rok Romih, Peter Veranič
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引用次数: 0

摘要

间质性膀胱炎/膀胱疼痛综合征(IC/BPS)是一种慢性膀胱炎症性疾病,由于既没有诊断标准,也没有可靠的非侵入性疾病生物标志物,因此对其进行正确诊断是一项挑战。本研究的目的是同时确定匹配的血清和尿液来源的IC/BPS生物标志物,这将为疾病机制提供额外的见解,并为进一步的生物标志物验证奠定基础。我们的研究包括12名女性IC/BPS患者和12名健康对照。总共测量了33种不同的生物标志物,包括细胞因子和趋化因子,参与细胞外基质重塑的蛋白质,粘附分子,生长因子,以及使用酶联免疫测定和多重技术的氧化应激标志物。热图和基于显著改变的生物标志物的主成分分析揭示了尿和血清相关的IC/BPS特征,明确区分IC/BPS患者与对照组。与对照组相比,IC/BPS患者血清和尿液中的四种生物标志物,包括CCL11、BAFF、HGF和MMP9,均显著上调。血清MMP9水平与疾病严重程度相关,可以很好地区分有无Hunner病变的IC/BPS患者。因此,MMP9的全身水平可以反映IC/BPS患者膀胱内的局部病理,MMP9可能被证明是开发新型治疗干预措施的有用靶点。利用尿液和血清生物标志物的综合小组,在这里确定,为IC/BPS患者的疾病检测带来了希望。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Matched serum- and urine-derived biomarkers of interstitial cystitis/bladder pain syndrome.

Matched serum- and urine-derived biomarkers of interstitial cystitis/bladder pain syndrome.

Matched serum- and urine-derived biomarkers of interstitial cystitis/bladder pain syndrome.

Matched serum- and urine-derived biomarkers of interstitial cystitis/bladder pain syndrome.

Setting up the correct diagnosis of interstitial cystitis/bladder pain syndrome (IC/BPS), a chronic inflammatory disease of the bladder, is a challenge, as there are neither diagnostic criteria nor reliable and non-invasive disease biomarkers available. The aim of the present study was to simultaneously determine matched serum- and urine-derived biomarkers of IC/BPS, which would provide additional insights into disease mechanisms and set the basis for further biomarker validation. Our study included 12 female patients with IC/BPS and 12 healthy controls. A total of 33 different biomarkers were measured, including cytokines and chemokines, proteins involved in extracellular matrix remodeling, adhesion molecules, growth factors, and markers of oxidative stress using enzyme linked immunoassays and multiplex technology. Heatmaps and principal component analysis based on significantly altered biomarkers, revealed urine- and serum-associated IC/BPS signatures that clearly differentiated IC/BPS patients from controls. Four biomarkers, including CCL11, BAFF, HGF and MMP9, were significantly upregulated in both serum and urine of patients with IC/BPS compared to controls. Serum levels of MMP9 were associated with disease severity and could distinguish well between IC/BPS patients with and without Hunner's lesions. Systemic levels of MMP9 can therefore mirror the local pathology within the bladders of IC/BPS patients, and MMP9 may prove to be a useful target for the development of novel therapeutic interventions. Utilizing a comprehensive panel of both urine and serum biomarkers, identified here, holds promise for disease detection in IC/BPS patients.

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来源期刊
PLoS ONE
PLoS ONE 生物-生物学
CiteScore
6.20
自引率
5.40%
发文量
14242
审稿时长
3.7 months
期刊介绍: PLOS ONE is an international, peer-reviewed, open-access, online publication. PLOS ONE welcomes reports on primary research from any scientific discipline. It provides: * Open-access—freely accessible online, authors retain copyright * Fast publication times * Peer review by expert, practicing researchers * Post-publication tools to indicate quality and impact * Community-based dialogue on articles * Worldwide media coverage
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