颌骨纤维骨化病变中硬骨素与肥大细胞之间的相互作用

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2024-12-30 DOI:10.1111/odi.15232

Riéli Elis Schulz, Michel Calil Abrão-Neto, Thiago Pires Claudio, Vinícius Gonçalves de Souza, Elena Riet Correa Rivero, Rogério Oliveira Gondak, Gustavo Davi Rabelo

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引用次数: 0

摘要

目的：探讨颌骨纤维骨性病变（FOL）组织中硬化蛋白、β-连环蛋白和胰蛋白酶的表达。对象和方法：在FOL和非病变骨上对这些蛋白进行免疫组化分析。硬化蛋白阳性细胞评分从0（无表达）到3（高表达）。结果：我们分析了46例FOL活检，并选择了38例患者。分类：纤维结构不良（FD） 15例，幼年小梁骨化纤维瘤（JTOF） 8例，沙粒样骨化纤维瘤（PsOF） 2例，FOL 13例。我们发现纤维组织中的硬化蛋白阳性细胞比骨骼中的多，表现出类似肥大细胞的表型，具有很强的点细胞质阳性。对硬化蛋白阳性细胞病变（评分为2和3）的分析显示，21例活检中80.9%的患者胰蛋白酶呈阳性。β-catenin在纤维组分上弥漫性表达，以胞浆染色为主。非病变骨在髓腔和少量骨细胞中可见硬化蛋白表达。结论：硬化蛋白阳性细胞多见于FOL的纤维组织，大多数硬化蛋白阳性病变中均存在胰蛋白酶肥大细胞标记物。

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Interaction Between Sclerostin and Mast Cells in Fibro-Osseous Lesions of the Jaws.

Objective: To assess the sclerostin, β-catenin, and tryptase expression in fibro-osseous lesions (FOL) of the jaws.

Subjects and methods: Immunohistochemistry analysis was performed for these proteins on FOL and non-lesional bone. The sclerostin-positive cells were scored from 0 (no expression) to 3 (high expression).

Results: We analyzed 46 FOL biopsies and selected 38 patients. Categorization showed 15 fibrous dysplasia (FD), eight juvenile trabecular ossifying fibroma (JTOF), two psammomatoid ossifying fibroma (PsOF), and 13 FOL. We found more sclerostin-positive cells in fibrous tissue than in bone, showing a phenotype like mast cells with strong dot-cytoplasmic positivity. The analysis of sclerostin-positive cell lesions (scored as 2 and 3) showed also tryptase positivity in 80.9% of 21 biopsies. β-catenin was diffusely expressed on the fibrous component, mostly with cytosol staining. Non-lesional bone showed sclerostin expression in medullary spaces and a few osteocytes.

Conclusions: Sclerostin-positive cells are mostly found in the fibrous tissue of FOL, and the tryptase mast cell marker was present in most of the lesions that were positive for sclerostin.

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.