Dietary chlorogenic acid supplementation protects against lipopolysaccharide-induced oxidative stress, inflammation and apoptosis in intestine of amur ide (Leuciscus waleckii)

In this study, the alleviative effects of chlorogenic acid (CGA) on oxidative stress, inflammation and apoptosis of amur ide (Leuciscus waleckii) induced by lipopolysaccharide (LPS) were evaluated. Using a 2 × 2 factorial design, amur ide were irregularity divided into 4 groups and fed two diets with 0.00 % (CK and LPS), 0.04 % CGA(CGA and LC). After 4-week feeding trial, LPS challenge was executed. Results showed that 0.04 % CGA alleviated LPS-induced intestinal barrier dysfunction by decreasing the levels of 5-HT, D-LA, ET-1 and DAO in serum, increasing ZO-1, Occludin-α, Claudin-c, Claudin-f mRNA, and ZO-1, Occludin, Claudin-1 protein expression, improving intestinal morphology. Moreover, 0.04 % CGA alleviated LPS-induced inflammation and apoptosis by up-regulating TGF-β and Bcl-2 mRNA, down-regulating NF-κBp65, TNF-α, Bax, Caspase-3, Caspase-9 mRNA and NF-κBp65, Bax, Caspase-3 protein expression. 0.04 %CGA reversed LPS-induced the reduction of GSH-PX, CAT, T-SOD and T-AOC in intestines, whereas MDA showed the opposite result. 0.04 % alleviated LPS-induced the decrease of Nrf2, HO-1, CAT, SOD mRNA and Nrf2 protein expression, the increase of Keap1 mRNA. Summary, this study suggested that 0.04 % of dietary CGA alleviated LPS-induced intestinal oxidative stress, inflammation and apoptosis of amur ide.