基于荧光的DNA编码文库合成中DNA损伤分析的简单实用方法。

IF 4 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Bioconjugate Chemistry Pub Date : 2025-03-19 Epub Date: 2024-12-31 DOI:10.1021/acs.bioconjchem.4c00483

Seungyoon Kang, Gyung A Kim, Myo Naing Win, Yeongcheol Ki, Hohjai Lee, Min Su Han

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引用次数: 0

摘要

建立了DNA编码文库（DEL）合成的DNA选择性荧光染料DNA损伤检测方法。针对不同的DEL合成条件，通过与高效液相色谱（HPLC）分析交叉验证，证实了使用特定比例的有机溶剂可严重诱导DNA损伤的事实。同时，通过筛选dna上酰胺偶联反应和pd催化的dna上n-芳基化反应的dna损伤条件，验证了该方法的适用性。

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Fluorescence-Based Simple and Practical Assay Method for DNA Damage Analysis in DNA-Encoded Library Synthesis.

The dsDNA-selective fluorescent-dye-based DNA damage assay was developed for DNA-encoded library (DEL) synthesis. For the various DEL synthesis conditions, the assay was validated through cross-checking with high-performance liquid chromatography (HPLC) analysis, and the fact was confirmed that the usage of a specific ratio of organic solvent can critically induce DNA damage. Also, the applicability of the assay was confirmed through the screening of the DNA-damaging condition of the on-DNA amide coupling reaction and Pd-catalyzed on-DNA N-arylation reaction.

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来源期刊

Bioconjugate Chemistry 生物-化学综合

CiteScore

9.00

自引率

2.10%

发文量

236

审稿时长

1.4 months

期刊介绍： Bioconjugate Chemistry invites original contributions on all research at the interface between man-made and biological materials. The mission of the journal is to communicate to advances in fields including therapeutic delivery, imaging, bionanotechnology, and synthetic biology. Bioconjugate Chemistry is intended to provide a forum for presentation of research relevant to all aspects of bioconjugates, including the preparation, properties and applications of biomolecular conjugates.