能量代谢、抗氧化防御系统、金属转运和离子稳态是野生稻SSSL耐Cd的关键因素

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials Pub Date : 2024-12-31 DOI:10.1016/j.jhazmat.2024.137009

Humera Ashraf , Fozia Ghouri , Lixia Sun , Weiwei Xia , Sawaira Ashraf , Muhammad Zahid Ashraf , Xuelin Fu , Shafaqat Ali , Muhammad Qasim Shahid

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引用次数: 0

摘要

镉（Cd）毒性对水稻种植构成重大挑战，影响植物的生长发育。野生水稻和纳米颗粒为提高Cd耐受性提供了有希望的策略，但对它们的联合效应知之甚少。本研究比较了野生稻单段代换系SG004与栽培稻（HJX74）对Cd胁迫的响应。两种基因型均用氧化钙纳米颗粒（np-CaO）处理。结果表明，Cd暴露破坏了这两个品系的活性氧（ROS）代谢，如丙二醛（MDA）在HJX74中比SG004增加了57%。此外，SG004的茎长比HJX74减少了26%，叶绿素ab含量比HJX74减少了42%，而HJX74减少了53%。在镉中毒条件下，HJX74的抗氧化活性如谷胱甘肽（GSH）比SG004降低了25%。此外，SG004的根Cd积累量（70%）和茎Cd积累量（85%）均低于HJX74，表明其对Cd毒性的耐受性增强。根细胞细胞学显示HJX74的不同细胞器有几种变形，而SG004的变形较少。RNAseq分析确定了关键途径，包括能量代谢、抗氧化防御、金属运输和离子稳态，这可能是SG004增强耐受性的关键。值得注意的是，两个不同的金属硫蛋白样基因（BGIOSGA019338, BGIOSGA035982），过氧化物酶（BGIOSGA019133），铵（BGIOSGA008640, BGIOSGA008641）和钾转运蛋白（BGIOSGA030867）， NRAMP1 （BGIOSGA025476）和铝激活的苹果酸转运蛋白（BGIOSGA014531）在Cd胁迫下在SG004中表现出差异表达。取代片段内的基因，包括过氧化物酶25 （BGIOSGA002866）、金属硫蛋白（BGIOSGA002389）和还原酶（BGIOSGA002387）的基因，也在SG004中上调，加强了抗氧化和离子稳态途径的作用。利用np-CaO可以缓解两种基因型的Cd诱导胁迫，从而加强了野生稻和纳米颗粒在提高Cd耐受性方面的应用。

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Energy metabolism, antioxidant defense system, metal transport, and ion homeostasis are key contributors to Cd tolerance in SSSL derived from wild rice

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Energy metabolism, antioxidant defense system, metal transport, and ion homeostasis are key contributors to Cd tolerance in SSSL derived from wild rice

Cadmium (Cd) toxicity poses major challenges to rice cultivation, affecting plant growth and development. Wild rice and nanoparticles offer promising strategies to enhance Cd tolerance, yet little is known about their combined effects. This study evaluates the single segment substitution line (SG004) from Oryza glumaepatula (wild rice) and its response to Cd stress compared to cultivated rice (HJX74). Both genotypes were also treated with calcium oxide nanoparticles (np-CaO). Results showed that Cd exposure disrupts reactive oxygen species (ROS) metabolism in both lines, such as malondialdehyde (MDA) increases by 57 % in HJX74 compared to SG004. Moreover, SG004 exhibited a 26 % reduction in shoot length compared to 41 % in HJX74 and a 42 % decline in chlorophyll ab content versus 53 % in HJX74. Antioxidant activity such as glutathione (GSH) decreased 25 % more in HJX74 than SG004 under Cd toxicity. Additionally, SG004 had lower Cd accumulation in roots (70 %) and shoots (85 %) than HJX74, indicating its enhanced tolerance to Cd toxicity. The root cell cytology reveals several deformations in different organelles of HJX74 but less in SG004. RNAseq analysis identifies key pathways, including energy metabolism, antioxidant defense, metal transport, and ion homeostasis, which may be critical for SG004 enhanced tolerance. Notably, two distinct metallothionein-like genes (BGIOSGA019338, BGIOSGA035982), a peroxidase (BGIOSGA019133), ammonium (BGIOSGA008640, BGIOSGA008641, and potassium transporters (BGIOSGA030867), NRAMP1 (BGIOSGA025476), and an aluminum-activated malate transporter (BGIOSGA014531), showed differential expressions in SG004 under Cd stress. Genes within the substituted fragment, including those for peroxidase 25 (BGIOSGA002866), metallothionein (BGIOSGA002389), and reductase (BGIOSGA002387), are also upregulated in SG004, reinforcing the role of antioxidant and ion homeostasis pathways. The utilization of np-CaO alleviates Cd-induced stress in both genotypes, hence reinforcing the application of wild rice and nanoparticles to improve Cd tolerance.

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来源期刊

Journal of Hazardous Materials 工程技术-工程：环境

CiteScore

25.40

自引率

5.90%

发文量

3059

审稿时长

58 days

期刊介绍： The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.