{"title":"从伊朗亚兹德省的传统乳制品厂和收集的原料奶中分离出的沙门氏菌属的频率和抗生素耐药性模式。","authors":"Raziyeh Barzegar-Bafrouei, Bahador Hajimohammadi, Hengameh Zandi, Gilda Eslami, Hossein Fallahzadeh","doi":"10.18502/ijm.v16i6.17248","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and objectives: </strong>Salmonellosis is among the most common food-born infections, caused by <i>Salmonella</i> spp. bacteria. Present study has investigated the frequency and antibiotic resistance pattern of <i>Salmonella</i> spp. isolated from traditional dairy products and raw milk supplied in Yazd, Iran.</p><p><strong>Materials and methods: </strong>In a cross-sectional study, 350 samples of raw milk and traditional dairy products were randomly collected from July to September 2018. Following culturing the samples, isolates went through biochemical tests for phenotypic identification. Results were confirmed through PCR technique by targeting <i>invA</i> gene. Antimicrobial susceptibility test was conducted by means of disk diffusion method.</p><p><strong>Results: </strong>The rate of contamination with <i>Salmonella</i> bacteria was 6.57% in all samples. The PCR assay of all isolates showed that 23 isolates (100%) carried the <i>invA</i> gene. No significant association between the frequency of <i>salmonella</i> spp. and types of dairy and their origin was reported (<i>P</i>>0.05). The highest antibiotic resistance rate among the isolates belonged to tetracycline (34.8%) and the highest sensitivity was seen to imipenem, cefepime, and cefotaxime (each 91.3%).</p><p><strong>Conclusion: </strong>According to our results there has been a rise in multiple drug resistance and contamination rate in traditional dairy products in Yazd province.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 6","pages":"755-762"},"PeriodicalIF":1.3000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11682549/pdf/","citationCount":"0","resultStr":"{\"title\":\"Frequency and antibiotic resistance pattern of <i>Salmonella</i> spp. isolated from traditional dairies and raw milks collected in Yazd province, Iran.\",\"authors\":\"Raziyeh Barzegar-Bafrouei, Bahador Hajimohammadi, Hengameh Zandi, Gilda Eslami, Hossein Fallahzadeh\",\"doi\":\"10.18502/ijm.v16i6.17248\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and objectives: </strong>Salmonellosis is among the most common food-born infections, caused by <i>Salmonella</i> spp. bacteria. Present study has investigated the frequency and antibiotic resistance pattern of <i>Salmonella</i> spp. isolated from traditional dairy products and raw milk supplied in Yazd, Iran.</p><p><strong>Materials and methods: </strong>In a cross-sectional study, 350 samples of raw milk and traditional dairy products were randomly collected from July to September 2018. Following culturing the samples, isolates went through biochemical tests for phenotypic identification. Results were confirmed through PCR technique by targeting <i>invA</i> gene. Antimicrobial susceptibility test was conducted by means of disk diffusion method.</p><p><strong>Results: </strong>The rate of contamination with <i>Salmonella</i> bacteria was 6.57% in all samples. The PCR assay of all isolates showed that 23 isolates (100%) carried the <i>invA</i> gene. No significant association between the frequency of <i>salmonella</i> spp. and types of dairy and their origin was reported (<i>P</i>>0.05). The highest antibiotic resistance rate among the isolates belonged to tetracycline (34.8%) and the highest sensitivity was seen to imipenem, cefepime, and cefotaxime (each 91.3%).</p><p><strong>Conclusion: </strong>According to our results there has been a rise in multiple drug resistance and contamination rate in traditional dairy products in Yazd province.</p>\",\"PeriodicalId\":14633,\"journal\":{\"name\":\"Iranian Journal of Microbiology\",\"volume\":\"16 6\",\"pages\":\"755-762\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11682549/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/ijm.v16i6.17248\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v16i6.17248","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Frequency and antibiotic resistance pattern of Salmonella spp. isolated from traditional dairies and raw milks collected in Yazd province, Iran.
Background and objectives: Salmonellosis is among the most common food-born infections, caused by Salmonella spp. bacteria. Present study has investigated the frequency and antibiotic resistance pattern of Salmonella spp. isolated from traditional dairy products and raw milk supplied in Yazd, Iran.
Materials and methods: In a cross-sectional study, 350 samples of raw milk and traditional dairy products were randomly collected from July to September 2018. Following culturing the samples, isolates went through biochemical tests for phenotypic identification. Results were confirmed through PCR technique by targeting invA gene. Antimicrobial susceptibility test was conducted by means of disk diffusion method.
Results: The rate of contamination with Salmonella bacteria was 6.57% in all samples. The PCR assay of all isolates showed that 23 isolates (100%) carried the invA gene. No significant association between the frequency of salmonella spp. and types of dairy and their origin was reported (P>0.05). The highest antibiotic resistance rate among the isolates belonged to tetracycline (34.8%) and the highest sensitivity was seen to imipenem, cefepime, and cefotaxime (each 91.3%).
Conclusion: According to our results there has been a rise in multiple drug resistance and contamination rate in traditional dairy products in Yazd province.
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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