17个新多核苷酸微卫星标记的鉴定与表征。

IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES

BMC Research Notes Pub Date : 2024-12-30 DOI:10.1186/s13104-024-07049-3

Ha Yeun Song, Young Ji Choi, Dea-Sung Lee, Hyun Ki Choi, Kyung Min Choi, Kang-Yeol Yu, Namki Cho, Seungki Lee

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引用次数: 0

摘要

摘要目的：韩国鬼蟹（十足目）因其数量减少而被指定为海洋保护物种。本研究通过新一代测序成功鉴定了17个微卫星标记。结果：在所检测的63个基因座中，26个在100个个体的样本中被有效扩增。这17个基因座包含4个三核苷酸重复序列和13个四核苷酸重复序列，在3个刺激稻种群的100个样本中，每个基因座显示4 ~ 26个等位基因（平均14.1个等位基因）。平均观察杂合度和期望杂合度分别为0.885和0.836。这17个新发现的多态微卫星位点对研究这一受保护海洋物种的遗传结构和多样性具有重要的实用价值。

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Identification and characterization of 17 novel polynucleotide microsatellite markers in Ocypode stimpsoni (Decapoda: Ocypodidae).

Objective: The ghost crab Ocypode stimpsoni (Decapoda) is designated as a protected marine species in Korea due to its declining population. In this study, we successfully identified 17 microsatellite markers for O. stimpsoni through next-generation sequencing.

Results: Out of the 63 loci examined, 26 were effectively amplified in a sample of 100 individuals. These 17 loci, comprising four tri-nucleotide and 13 tetra-nucleotide repeats, exhibited a range of 4 to 26 alleles per locus (with an average of 14.1 alleles) across 100 samples from three O. stimpsoni populations. The mean observed and expected heterozygosities were calculated to be 0.885 and 0.836, respectively. These 17 newly identified polymorphic microsatellite loci hold valuable utility for investigating the genetic structure and diversity of this protected marine species.

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来源期刊

BMC Research Notes Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.60

自引率

0.00%

发文量

363

审稿时长

15 weeks

期刊介绍： BMC Research Notes publishes scientifically valid research outputs that cannot be considered as full research or methodology articles. We support the research community across all scientific and clinical disciplines by providing an open access forum for sharing data and useful information; this includes, but is not limited to, updates to previous work, additions to established methods, short publications, null results, research proposals and data management plans.