γ-氨基丁酸和n -甲基- d -天冬氨酸受体参与糖尿病大鼠胃病:长期胰岛素和镁补充治疗可能的有益作用。

Q3 Veterinary
Archives of Razi Institute Pub Date : 2024-06-30 eCollection Date: 2024-06-01 DOI:10.32592/ARI.2024.79.3.601
H Saberi, N Mehranfard, H Rezazadeh, M Ghasemi
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引用次数: 0

摘要

胃肠功能障碍是糖尿病患者严重而常见的并发症。一些证据表明γ -氨基丁酸(GABA)和谷氨酸有助于糖尿病胃肠道异常。因此,我们研究了长期使用胰岛素和镁补充剂对2型糖尿病大鼠胃中GABA A型(GABA-A)、GABA- b和n -甲基- d -天冬氨酸(NMDA)谷氨酸受体以及一氧化氮合酶1 (NOS-1)表达模式的影响。24只雄性Wistar大鼠随机分为4组(每组6只):1)对照组,2)2型糖尿病:高脂饮食3个月+低剂量链脲佐菌素(35 mg/kg), 3) 2型糖尿病+镁,4)2型糖尿病+胰岛素。western blotting检测NOS-1、GABA-A、GABA-B和NMDA受体的表达。NOS-1表达明显降低(P
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Involvement of γ-Aminobutyric Acid and N-methyl-D-aspartate Receptors in Diabetic Gastropathy in Rats: Possible Beneficial Effect of Prolonged Treatment with Insulin and Magnesium Supplement.

Gastrointestinal dysfunction is a severe and common complication in diabetic patients. Some evidence shows that gamma-aminobutyric acid (GABA) and glutamate contribute to diabetic gastrointestinal abnormalities. Therefore, we examined the impact of prolonged treatment with insulin and magnesium supplements on the expression pattern of GABA type A (GABA-A), GABA-B, and N-methyl-D-aspartate (NMDA) glutamate receptors as well as nitric oxide synthase 1 (NOS-1) in the stomach of type 2 diabetic rats. Twenty-four male Wistar rats were randomized to four groups (six rats each): 1) control, 2) type 2 diabetes: rats fed with a high-fat diet for three months + a low dose of streptozotocin (35 mg/kg), 3) type 2 diabetes + magnesium, and 4) type 2 diabetes + insulin. The expression of NOS-1, GABA-A, GABA-B, and NMDA receptors was detected using western blotting. The NOS-1 expression was substantially diminished (P<0.01), while the expression of GABA-A (P<0.001), GABA-B (P<0.001), and NMDA (P<0.001) receptors was enhanced in the stomach of diabetic rats relative to control. Treatment with magnesium and insulin improved NOS-1 expression in diabetic rats, although this effect was greater in magnesium treatment alone. Magnesium also restored the expression of GABA-A and GABA-B receptors in diabetic rats to control values. Moreover, insulin treatment improved GABA-A receptor expression in diabetic rats (P<0.05). No considerable alterations were detected in NMDA receptor levels in the treatment groups. The results suggest a significant role of magnesium and insulin in improving gastric motility and secretory disorders associated with diabetes through modifying the expression of GABAergic receptors.

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来源期刊
Archives of Razi Institute
Archives of Razi Institute Veterinary-Veterinary (all)
CiteScore
1.50
自引率
0.00%
发文量
108
审稿时长
12 weeks
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