Dose-responsive phytotoxicity and oxidative stress induced by metal–organic framework PCN-224 in Arabidopsis thaliana seedlings

Metal–organic frameworks (MOFs) are advanced porous materials composed of metal ions and organic ligands, known for their unique structures and fascinating physio-chemical properties. To ensure their safe production and applications, it is crucial to thoroughly investigate their toxicity and environmental hazards. However, the potential risks of MOFs, particularly their impact on plants remained underexplored. Herein, we systematically assessed the phytotoxicity of PCN-224 on Arabidopsis thaliana (A. thaliana) due to its commercial availability and widespread use. To achieve this goal, A. thaliana seedlings were subjected to PCN-224 concentrations (10–300 µg/mL) and durations (1–12 days) in agar media, with a control group. PCN-224 slightly accelerated seed germination across all concentrations without altering the total germination rate. Exposure to a higher concentration of PCN-224 (300 µg/mL) significantly impaired A. thaliana development, reducing fresh weight (54.0%) and root length (82.3%) compared with control; however, lower exposure (10 µg/mL) showed minimal growth inhibition. Fluorescence microscopy showed that PI-labeled PCN-224 particles adhered to root surfaces and internalized in a concentration- and time-dependent manner, with notable xylem accumulation after 2 h. The net photosynthetic rate, transpiration rate, and stomatal conductance decreased by 54.25%, 62.37% and 38.53%, respectively, compared with control, when the material concentration exceeded 100 µg/mL. Regarding the oxidative damage, higher PCN-224 exposure reduced antioxidant levels and downregulation of antioxidant-related genes resulted in a diminished oxidative stress response. Overall, our study highlights the potential risk of MOFs for plant growth and emphasizes the need to assess their environmental impact for sustainable agricultural practices.