低背景催化氧化还原循环耦合杂交链反应扩增高灵敏度电化学适体黄体生成素测定。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2024-12-26 DOI:10.1016/j.bioelechem.2024.108888

Yuan Ming , Yujie Liu , Daxiu Li , Bingying Jiang , Yun Xiang , Ruo Yuan

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引用次数: 0

摘要

黄体生成素（LH）的浓度变化调节卵母细胞减数分裂的细胞周期，显著影响整个生殖周期。因此，对LH生物标志物的敏感量化对生殖疾病的诊断具有重要作用。通过将低背景催化氧化还原回收策略与杂化链反应（HCR）相结合，提出了一种高灵敏度的生物电化学适体LH检测方法。LH分析物分子结合双链dna的适体链释放ssdna，触发传感器电极上的[Ru(NH3)6]Cl3 （RuHex）修饰的dsDNA聚合物HCR生成。随后由K3[Fe(CN)6]介导的RuHex的电化学氧化还原循环因此显示出超灵敏LH测定的极大放大电流。HCR信号放大与低背景氧化还原循环的协同集成，使检测LH的信噪比和灵敏度提高到6.03 pM。此外，在稀释的人血清中检测LH已经得到了测试和验证，使这种传感器成为一个强大的检测平台，可以监测低水平的多种生物标志物，用于疾病的早期诊断。

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Low background catalytic redox recycling coupled with hybridization chain reaction amplification for highly sensitive electrochemical aptamer luteinizing hormone assay

The concentration variation of luteinizing hormone (LH) regulates the cell cycle of oocyte meiosis and significantly affect the whole reproductive cycle. Sensitively quantifying the LH biomarker therefore plays an important role for reproductive disease diagnosis. By coupling a new low background catalytic redox recycling strategy with hybridization chain reaction (HCR), we propose a highly sensitive bio-electrochemical aptamer LH sensing method. LH analyte molecules bind aptamer strands in duplex DNAs to liberate ssDNAs, which trigger HCR generation of [Ru(NH₃)₆]Cl₃ (RuHex)-modified dsDNA polymers on sensor electrode. Subsequent electrochemical redox recycling of RuHex mediated by K₃[Fe(CN)₆] thus exhibits greatly magnified currents for ultrasensitive LH assay. The synergistic integration of HCR signal amplification with low background redox recycling leads to highly enhanced signal-to-noise ratio and sensitivity for detecting LH down to 6.03 pM. In addition, LH sensing in diluted human serums has been tested and verified, making such sensor a robust detection platform for monitoring diverse biomarkers at low levels for early diagnosing diseases.

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.