姜黄素对甲氨蝶呤诱导的大鼠卵巢损伤和卵泡储备的影响:PARP-1和P53的作用。

Annals of medicine Pub Date : 2025-12-01 Epub Date: 2024-12-27 DOI:10.1080/07853890.2024.2446688
Mete Keçeci, Nesibe Karaoluk
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引用次数: 0

摘要

背景:甲氨蝶呤(MTX)是一种用于治疗许多肿瘤性和非肿瘤性疾病的药物,已知会引起正常组织的氧化损伤。姜黄素是一种天然多酚化合物,具有强大的抗氧化和抗细胞凋亡作用。在这项研究中,我们研究了Cur对mtx诱导的卵巢损伤的影响。材料与方法:将32只成年雌性Wistar白化大鼠分为4组:(1)对照组(n = 8):只给药组,(2)Cur组(n = 8):只给药组(200 mg/kg/d), (3) MTX组(n = 8):只给药组(0.35 mg/kg/d), (4) MTX+Cur组(n = 8):分别给药MTX (0.35 mg/kg/d)和Cur (200 mg/kg/d),连续给药28 d。采用酶联免疫吸附法(ELISA)检测各组小鼠SOD、CAT、MDA、AMH水平。在H&E染色玻片上进行卵泡计数。免疫组化分析P53、PARP-1的表达。结果:MTX组MDA水平明显高于MTX+Cur组(p p p p p p p p p p p)结论:MTX给药前Cur预处理可能通过调节P53和PARP-1的表达及其抗氧化作用来保护卵巢卵泡池。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of curcumin on methotrexate-induced ovarian damage and follicle reserve in rats: the role of PARP-1 and P53.

Background: Methotrexate (MTX) is an agent used in the treatment of many neoplastic and non-neoplastic diseases and is known to cause oxidative damage in normal tissues. Curcumin (Cur) is a natural polyphenol compound with powerful antioxidant and antiapoptotic effects. In this study we investigate the effects of Cur on MTX-induced ovarian damage.

Materials and methods: Thirty-two young adult female Wistar albino rats were divided into four groups: (1) Control (n = 8): only vehicle group, (2) Cur (n = 8): Cur-only group (200 mg/kg/day), (3) MTX (n = 8): MTX-only group (0.35 mg/kg/day), (4) MTX+Cur (n = 8): The group was given MTX (0.35 mg/kg/day) and Cur (200 mg/kg/day) for 28 days. Then, SOD, CAT, MDA, AMH levels were measured using ELISA kits. Follicle count was performed on H&E stained slides. In addition, the expressions of P53 and PARP-1 were analysed by immunohistochemistry.

Results: MDA levels were seen to be higher in the MTX group than in the MTX+Cur group (p < 0.05). Cur treatment lowered MDA levels and increased SOD and CAT levels (p < 0.05 for all). In the MTX+Cur group, atretic follicle count decreased (p < 0,05), however, primordial follicle count increased (p < 0,01). Secondary follicle count and AMH levels were higher in MTX-treated groups (p < 0,05 and p < 0,01, respectively). Expressions of p53 and Poly [ADP-ribose] polymerase 1 (PARP-1) increased significantly in the MTX group compared to the other groups (p < 0,05).

Conclusion: Cur pretreatment prior to MTX administration may be an effective option in preserving the ovarian follicle pool by regulating P53 and PARP-1 expressions with its antioxidant effect.

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