{"title":"姜黄素对甲氨蝶呤诱导的大鼠卵巢损伤和卵泡储备的影响:PARP-1和P53的作用。","authors":"Mete Keçeci, Nesibe Karaoluk","doi":"10.1080/07853890.2024.2446688","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Methotrexate (MTX) is an agent used in the treatment of many neoplastic and non-neoplastic diseases and is known to cause oxidative damage in normal tissues. Curcumin (Cur) is a natural polyphenol compound with powerful antioxidant and antiapoptotic effects. In this study we investigate the effects of Cur on MTX-induced ovarian damage.</p><p><strong>Materials and methods: </strong>Thirty-two young adult female Wistar albino rats were divided into four groups: (1) Control (<i>n</i> = 8): only vehicle group, (2) Cur (<i>n</i> = 8): Cur-only group (200 mg/kg/day), (3) MTX (<i>n</i> = 8): MTX-only group (0.35 mg/kg/day), (4) MTX+Cur (<i>n</i> = 8): The group was given MTX (0.35 mg/kg/day) and Cur (200 mg/kg/day) for 28 days. Then, SOD, CAT, MDA, AMH levels were measured using ELISA kits. Follicle count was performed on H&E stained slides. In addition, the expressions of P53 and PARP-1 were analysed by immunohistochemistry.</p><p><strong>Results: </strong>MDA levels were seen to be higher in the MTX group than in the MTX+Cur group (<i>p</i> < 0.05). Cur treatment lowered MDA levels and increased SOD and CAT levels (<i>p</i> < 0.05 for all). In the MTX+Cur group, atretic follicle count decreased (<i>p</i> < 0,05), however, primordial follicle count increased (<i>p</i> < 0,01). Secondary follicle count and AMH levels were higher in MTX-treated groups (<i>p</i> < 0,05 and <i>p</i> < 0,01, respectively). Expressions of p53 and Poly [ADP-ribose] polymerase 1 (PARP-1) increased significantly in the MTX group compared to the other groups (<i>p</i> < 0,05).</p><p><strong>Conclusion: </strong>Cur pretreatment prior to MTX administration may be an effective option in preserving the ovarian follicle pool by regulating P53 and PARP-1 expressions with its antioxidant effect.</p>","PeriodicalId":93874,"journal":{"name":"Annals of medicine","volume":"57 1","pages":"2446688"},"PeriodicalIF":0.0000,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of curcumin on methotrexate-induced ovarian damage and follicle reserve in rats: the role of PARP-1 and P53.\",\"authors\":\"Mete Keçeci, Nesibe Karaoluk\",\"doi\":\"10.1080/07853890.2024.2446688\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Methotrexate (MTX) is an agent used in the treatment of many neoplastic and non-neoplastic diseases and is known to cause oxidative damage in normal tissues. Curcumin (Cur) is a natural polyphenol compound with powerful antioxidant and antiapoptotic effects. In this study we investigate the effects of Cur on MTX-induced ovarian damage.</p><p><strong>Materials and methods: </strong>Thirty-two young adult female Wistar albino rats were divided into four groups: (1) Control (<i>n</i> = 8): only vehicle group, (2) Cur (<i>n</i> = 8): Cur-only group (200 mg/kg/day), (3) MTX (<i>n</i> = 8): MTX-only group (0.35 mg/kg/day), (4) MTX+Cur (<i>n</i> = 8): The group was given MTX (0.35 mg/kg/day) and Cur (200 mg/kg/day) for 28 days. Then, SOD, CAT, MDA, AMH levels were measured using ELISA kits. Follicle count was performed on H&E stained slides. In addition, the expressions of P53 and PARP-1 were analysed by immunohistochemistry.</p><p><strong>Results: </strong>MDA levels were seen to be higher in the MTX group than in the MTX+Cur group (<i>p</i> < 0.05). Cur treatment lowered MDA levels and increased SOD and CAT levels (<i>p</i> < 0.05 for all). In the MTX+Cur group, atretic follicle count decreased (<i>p</i> < 0,05), however, primordial follicle count increased (<i>p</i> < 0,01). Secondary follicle count and AMH levels were higher in MTX-treated groups (<i>p</i> < 0,05 and <i>p</i> < 0,01, respectively). Expressions of p53 and Poly [ADP-ribose] polymerase 1 (PARP-1) increased significantly in the MTX group compared to the other groups (<i>p</i> < 0,05).</p><p><strong>Conclusion: </strong>Cur pretreatment prior to MTX administration may be an effective option in preserving the ovarian follicle pool by regulating P53 and PARP-1 expressions with its antioxidant effect.</p>\",\"PeriodicalId\":93874,\"journal\":{\"name\":\"Annals of medicine\",\"volume\":\"57 1\",\"pages\":\"2446688\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annals of medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/07853890.2024.2446688\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/12/27 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/07853890.2024.2446688","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/27 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
背景:甲氨蝶呤(MTX)是一种用于治疗许多肿瘤性和非肿瘤性疾病的药物,已知会引起正常组织的氧化损伤。姜黄素是一种天然多酚化合物,具有强大的抗氧化和抗细胞凋亡作用。在这项研究中,我们研究了Cur对mtx诱导的卵巢损伤的影响。材料与方法:将32只成年雌性Wistar白化大鼠分为4组:(1)对照组(n = 8):只给药组,(2)Cur组(n = 8):只给药组(200 mg/kg/d), (3) MTX组(n = 8):只给药组(0.35 mg/kg/d), (4) MTX+Cur组(n = 8):分别给药MTX (0.35 mg/kg/d)和Cur (200 mg/kg/d),连续给药28 d。采用酶联免疫吸附法(ELISA)检测各组小鼠SOD、CAT、MDA、AMH水平。在H&E染色玻片上进行卵泡计数。免疫组化分析P53、PARP-1的表达。结果:MTX组MDA水平明显高于MTX+Cur组(p p p p p p p p p p p)结论:MTX给药前Cur预处理可能通过调节P53和PARP-1的表达及其抗氧化作用来保护卵巢卵泡池。
Effect of curcumin on methotrexate-induced ovarian damage and follicle reserve in rats: the role of PARP-1 and P53.
Background: Methotrexate (MTX) is an agent used in the treatment of many neoplastic and non-neoplastic diseases and is known to cause oxidative damage in normal tissues. Curcumin (Cur) is a natural polyphenol compound with powerful antioxidant and antiapoptotic effects. In this study we investigate the effects of Cur on MTX-induced ovarian damage.
Materials and methods: Thirty-two young adult female Wistar albino rats were divided into four groups: (1) Control (n = 8): only vehicle group, (2) Cur (n = 8): Cur-only group (200 mg/kg/day), (3) MTX (n = 8): MTX-only group (0.35 mg/kg/day), (4) MTX+Cur (n = 8): The group was given MTX (0.35 mg/kg/day) and Cur (200 mg/kg/day) for 28 days. Then, SOD, CAT, MDA, AMH levels were measured using ELISA kits. Follicle count was performed on H&E stained slides. In addition, the expressions of P53 and PARP-1 were analysed by immunohistochemistry.
Results: MDA levels were seen to be higher in the MTX group than in the MTX+Cur group (p < 0.05). Cur treatment lowered MDA levels and increased SOD and CAT levels (p < 0.05 for all). In the MTX+Cur group, atretic follicle count decreased (p < 0,05), however, primordial follicle count increased (p < 0,01). Secondary follicle count and AMH levels were higher in MTX-treated groups (p < 0,05 and p < 0,01, respectively). Expressions of p53 and Poly [ADP-ribose] polymerase 1 (PARP-1) increased significantly in the MTX group compared to the other groups (p < 0,05).
Conclusion: Cur pretreatment prior to MTX administration may be an effective option in preserving the ovarian follicle pool by regulating P53 and PARP-1 expressions with its antioxidant effect.