漆酶功能化zno -聚苯胺纳米复合材料电化学检测十六烷基三甲基溴化铵(CTAB)

IF 6.8 Q1 TOXICOLOGY
Hilda Dinah Kyomuhimbo, Usisipho Feleni, Nils Hendrik Haneklaus, Hendrik Gideon Brink
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引用次数: 0

摘要

由于阳离子表面活性剂在许多产品中的广泛应用,其直接排放到环境基质中的数量呈指数增长。这些化合物及其降解产物破坏微生物动力学,阻碍植物生存,并影响人类健康。因此,迫切需要发展电分析评估技术来识别、测定和监测它们。在我们的研究中,将ZnO-PANI纳米复合材料电沉积在玻璃碳电极(GCE)上,然后固定化漆酶并电沉积聚吡咯(PPy),形成用于检测CTAB的生物传感器。紫外可见光谱分析显示,苯环和类醌环的π-π*跃迁、π-极化子跃迁和n-π*极化子跃迁与PANI的延伸线圈链构象有关,氧化锌与PANI和3型铜在漆酶中存在并相互作用。FTIR分析显示,胺的N-H和C- n拉伸和弯曲峰对应,共轭烯烃的C=C拉伸峰对应,芳香化合物的C- h弯曲峰对应。高分辨率扫描电镜(HRSEM)分析表明,聚苯胺和zno -聚苯胺是由于与漆酶的共价键作用而沉积成毛状结构的纤维。以改性电极(py -6/GCE)为检测平台,在0.5 ~ 100µM、200 ~ 500µM和700 ~ 1900µM三个线性范围内检测CTAB。该传感器对自来水和废水的检测灵敏度为0.935 μA μM-1 cm-2,检出限为0.0116µM,可接受回收率分别为95.02%和87.84%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ZnO-Polyaniline Nanocomposite Functionalised with Laccase Enzymes for Electrochemical Detection of Cetyltrimethylammonuium Bromide (CTAB).

The direct discharge of cationic surfactants into environmental matrices has exponentially increased due to their wide application in many products. These compounds and their degraded products disrupt microbial dynamics, hinder plant survival, and affect human health. Therefore, there is an urgent need to develop electroanalytical assessment techniques for their identification, determination, and monitoring. In our study, ZnO-PANI nanocomposites were electrodeposited on a glassy carbon electrode (GCE), followed by the immobilization of laccase enzymes and the electrodeposition of polypyrrole (PPy), to form a biosensor that was used for the detection of CTAB. A UV-Vis analysis showed bands corresponding to the π-π* transition of benzenoid and quinoid rings, π-polaron band transition and n-π*polaronic transitions associated with the extended coil chain conformation of PANI, and the presence and interaction of ZnO with PANI and type 3 copper in the laccase enzymes. The FTIR analysis exhibited peaks corresponding to N-H and C-N stretches and bends for amine, C=C stretches for conjugated alkenes, and a C-H bend for aromatic compounds. A high-resolution scanning electron microscopy (HRSEM) analysis proved that PANI and ZnO-PANI were deposited as fibres with hairy topography resulting from covalent bonding with the laccase enzymes. The modified electrode (PPy-6/GCE) was used as a platform for the detection of CTAB with three linear ranges of 0.5-100 µM, 200-500 µM, and 700-1900 µM. The sensor displayed a high sensitivity of 0.935 μA μM-1 cm-2, a detection limit of 0.0116 µM, and acceptable recoveries of 95.02% and 87.84% for tap water and wastewater, respectively.

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来源期刊
CiteScore
5.30
自引率
1.70%
发文量
21
审稿时长
10 weeks
期刊介绍: The Journal of Xenobiotics publishes original studies concerning the beneficial (pharmacology) and detrimental effects (toxicology) of xenobiotics in all organisms. A xenobiotic (“stranger to life”) is defined as a chemical that is not usually found at significant concentrations or expected to reside for long periods in organisms. In addition to man-made chemicals, natural products could also be of interest if they have potent biological properties, special medicinal properties or that a given organism is at risk of exposure in the environment. Topics dealing with abiotic- and biotic-based transformations in various media (xenobiochemistry) and environmental toxicology are also of interest. Areas of interests include the identification of key physical and chemical properties of molecules that predict biological effects and persistence in the environment; the molecular mode of action of xenobiotics; biochemical and physiological interactions leading to change in organism health; pathophysiological interactions of natural and synthetic chemicals; development of biochemical indicators including new “-omics” approaches to identify biomarkers of exposure or effects for xenobiotics.
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