超氧化物歧化酶(sod2)基因val16ala多态性与儿童通气肺活量的变化-放射性污染地区居民和战时应激生活事件影响的儿童。

I Ye Kolpakov, V M Zigalo, V H Kondrashova, V A Poznysh, L O Leonovych
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引用次数: 0

摘要

目的:了解战时放射性污染地区儿童和应激生活事件暴露儿童锰超氧化物歧化酶基因多态性的分布及通气肺活量的变化。材料和方法:研究对象为学龄儿童——放射性污染地区(RCA)的居民,以及战时暴露于压力生活事件中的儿童。所有接受检查的人都没有呼吸道病理的临床症状。采用聚合酶链反应(PCR)法测定SOD2 Val16Ala基因变异的基因型。采用计算机肺活量测定法,以流量-容量环分析数据为基础评价通气肺活量的研究。结果与结论:在研究多态标记超氧化物歧化酶2基因val16的基因型和等位基因时,I组和II组儿童的基因型和等位基因频率分布指标与由实际健康的中东居民组成的对照组的参考值相比,没有发现显著差异。此外,与其他高加索人种(立陶宛人、芬兰人、德国人)的代表相比,两组儿童的多态性标记超氧化物歧化酶2基因Val16Ala的C和T等位基因的频率分布没有显著差异。在I组和II组患儿中,CC基因型纯合子出现频率呈下降趋势,CT基因型杂合子出现频率呈上升趋势。在两个检查组的儿童中,与CC纯合子相比,在超氧化物歧化酶2基因Val16Ala多态性的CT基因型的杂合子中,观察到支气管高反应性频率增加的趋势。对两组儿童sod2 Val16Ala多态性变异等位基因频率分布的分析确定,在支气管高反应性存在的情况下,T等位基因的患病率有增加的趋势,而C等位基因的患病率有降低的趋势。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
SUPEROXIDE DISMUTASE (SOD2) GENE VAL16ALA POLYMORPHISM AND CHANGES IN THE VENTILATION LUNG CAPACITY OF CHILDREN - RESIDENTS OF RADIOACTIVELY CONTAMINATED TERRITORIES AND CHILDREN WHO HAVE BEEN AFFECTED BY STRESSFUL LIFE EVENTS DURING WARTIME.

Objective: to determine the distribution of genotypes of genetic polymorphism of manganese superoxide dismutaseand to assess the ventilation lung capacity in children- residents of radioactively contaminated areas and childrenexposed to stressful life events during the war period.

Materials and methods: The study involved school-age children - residents of radioactively contaminated areas(RCA) and children exposed to stressful life events during wartime. All those examined had no clinical signs of respiratory pathology. Genotypes for the SOD2 Val16Ala genetic variant were determined using the polymerase chainreaction (PCR) method. The study of the ventilation lung capacity was assessed by the method of computer spirometry based on the flow-volume loop analysis data.

Results and conclusions: When studying the genotypes and alleles of the polymorphic marker SuperoxideDismutase 2 Gene Val16Alain children of group I and II, no significant differences were found between the frequencydistribution indicators of genotypes and alleles compared with the reference values of the those of the controlgroup, which consisted of practically healthy residents of the Middle East. Also, no significant differences in the frequency distribution of the C and T alleles of the polymorphic marker Superoxide Dismutase 2 Gene Val16Ala werefound in the children of both examined groups compared to other representatives of the Caucasian race (Lithuanians, Finns, Germans). Among children in groups I and II, there was a tendency toward a decrease in the frequency of occurrence of homozygotes with the CC genotype and an increase in the frequency of occurrence of heterozygotes with the CT genotype. Among children of both examined groups, in heterozygotes with the CT genotype of theSuperoxide Dismutase 2 Gene Val16Ala polymorphism, a tendency towards an increase in the frequency of bronchialhyperreactivity was observed compared to CC homozygotes. Analysis of the variant allele frequency distribution ofSOD2 Val16Ala polymorphism in children of both examined groups determined that in the presence of bronchial hyperreactivity there was a tendency toward an increase in the prevalence of the T allele and a decrease in the prevalence of the C allele.

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Problemy radiatsiinoi medytsyny ta radiobiolohii
Problemy radiatsiinoi medytsyny ta radiobiolohii Medicine-Radiology, Nuclear Medicine and Imaging
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