{"title":"甘氨酸甜菜碱积累通过CsBADH过表达增强茶树耐寒性","authors":"Yuqi Zhou, Lifan Guo, Zhenbin Chen, Peiqiang Wang, Xinfu Zhang, Lei Zhao","doi":"10.1016/j.plaphy.2024.109454","DOIUrl":null,"url":null,"abstract":"<p><p>Cold stress significantly limits the growth and yield of tea plants (Camellia sinensis (L.) O. Kuntze), particularly in northern China, may lead to huge economic losses. Glycine betaine (GB), an osmotic regulator, is widely applied in crop resistance to abiotic stress. This study investigates the role of GB and its biosynthetic enzyme CsBADH in enhancing cold tolerance in tea plants. Two cultivars, 'Shuchazao' (cold-resistant) and 'Baiye 1' (cold-sensitive), were subjected to low temperature stress (0 °C). GB accumulation was measured, revealing that 'Shuchazao' exhibited 1.4-fold higher GB levels than 'Baiye 1', suggesting a link between higher GB accumulation and cold tolerance. Exogenous GB treatment improved cold resistance, especially in the cold-sensitive cultivar 'Baiye 1'. The CsBADH gene, a key enzyme in GB biosynthesis, was cloned and expressed in Escherichia coli, confirming its activity. Transgenic Arabidopsis thaliana, Nicotiana tabacum, and C. sinensis plants overexpressing CsBADH showed increased GB levels (1.5- to 2.4-fold), proline content, peroxidase (POD) activities, and enhanced cold tolerance, while silencing CsBADH decreased GB accumulation and cold resistance. These findings demonstrate that CsBADH plays a critical role in cold stress response by promoting GB accumulation, offering potential strategies for improving the resilience of tea and other leaf crops to cold stress.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109454"},"PeriodicalIF":6.1000,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhancement of cold tolerance in tea plants (Camellia sinensis) by glycine betaine accumulation through CsBADH overexpression.\",\"authors\":\"Yuqi Zhou, Lifan Guo, Zhenbin Chen, Peiqiang Wang, Xinfu Zhang, Lei Zhao\",\"doi\":\"10.1016/j.plaphy.2024.109454\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Cold stress significantly limits the growth and yield of tea plants (Camellia sinensis (L.) O. Kuntze), particularly in northern China, may lead to huge economic losses. Glycine betaine (GB), an osmotic regulator, is widely applied in crop resistance to abiotic stress. This study investigates the role of GB and its biosynthetic enzyme CsBADH in enhancing cold tolerance in tea plants. Two cultivars, 'Shuchazao' (cold-resistant) and 'Baiye 1' (cold-sensitive), were subjected to low temperature stress (0 °C). GB accumulation was measured, revealing that 'Shuchazao' exhibited 1.4-fold higher GB levels than 'Baiye 1', suggesting a link between higher GB accumulation and cold tolerance. Exogenous GB treatment improved cold resistance, especially in the cold-sensitive cultivar 'Baiye 1'. The CsBADH gene, a key enzyme in GB biosynthesis, was cloned and expressed in Escherichia coli, confirming its activity. Transgenic Arabidopsis thaliana, Nicotiana tabacum, and C. sinensis plants overexpressing CsBADH showed increased GB levels (1.5- to 2.4-fold), proline content, peroxidase (POD) activities, and enhanced cold tolerance, while silencing CsBADH decreased GB accumulation and cold resistance. These findings demonstrate that CsBADH plays a critical role in cold stress response by promoting GB accumulation, offering potential strategies for improving the resilience of tea and other leaf crops to cold stress.</p>\",\"PeriodicalId\":20234,\"journal\":{\"name\":\"Plant Physiology and Biochemistry\",\"volume\":\"219 \",\"pages\":\"109454\"},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2024-12-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Physiology and Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1016/j.plaphy.2024.109454\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.plaphy.2024.109454","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Enhancement of cold tolerance in tea plants (Camellia sinensis) by glycine betaine accumulation through CsBADH overexpression.
Cold stress significantly limits the growth and yield of tea plants (Camellia sinensis (L.) O. Kuntze), particularly in northern China, may lead to huge economic losses. Glycine betaine (GB), an osmotic regulator, is widely applied in crop resistance to abiotic stress. This study investigates the role of GB and its biosynthetic enzyme CsBADH in enhancing cold tolerance in tea plants. Two cultivars, 'Shuchazao' (cold-resistant) and 'Baiye 1' (cold-sensitive), were subjected to low temperature stress (0 °C). GB accumulation was measured, revealing that 'Shuchazao' exhibited 1.4-fold higher GB levels than 'Baiye 1', suggesting a link between higher GB accumulation and cold tolerance. Exogenous GB treatment improved cold resistance, especially in the cold-sensitive cultivar 'Baiye 1'. The CsBADH gene, a key enzyme in GB biosynthesis, was cloned and expressed in Escherichia coli, confirming its activity. Transgenic Arabidopsis thaliana, Nicotiana tabacum, and C. sinensis plants overexpressing CsBADH showed increased GB levels (1.5- to 2.4-fold), proline content, peroxidase (POD) activities, and enhanced cold tolerance, while silencing CsBADH decreased GB accumulation and cold resistance. These findings demonstrate that CsBADH plays a critical role in cold stress response by promoting GB accumulation, offering potential strategies for improving the resilience of tea and other leaf crops to cold stress.
期刊介绍：
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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