Fabiano Palmeira Gonçalves, Olney Vieira-da-Motta, Pantaleo Scelza Neto, Solange Silva Samarão, Sandro Junio de Oliveira Tavares, Sérgio Henrique Seabra, Gabriel Bonan Taveira, Miriam Fatima Zaccaro Scelza
{"title":"探讨呋喃妥因在再生牙髓学中控制感染的潜力：体外研究。","authors":"Fabiano Palmeira Gonçalves, Olney Vieira-da-Motta, Pantaleo Scelza Neto, Solange Silva Samarão, Sandro Junio de Oliveira Tavares, Sérgio Henrique Seabra, Gabriel Bonan Taveira, Miriam Fatima Zaccaro Scelza","doi":"10.1016/j.joen.2024.12.009","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>This study assessed a new antimicrobial paste formulation containing nitrofurantoin for regenerative endodontics against multispecies biofilms.</p><p><strong>Methods: </strong>Four groups of 11 single-root teeth each were tested: Negative control group (brain-heart infusion broth + micro-organisms); Hoshino 1 conventional tri-antibiotic paste group (1:1:1 ratio); Hoshino 5 group (5:5:5 ratio); Experimental group with nitrofurantoin, ciprofloxacin, and metronidazole. Minimum inhibitory concentrations of each antimicrobial were determined. A polymicrobial biofilm (Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans) was created in root canals, and pastes were inserted for 3 minutes with lentulo. After 7 days, samples were sonicated and spectrophotometrically analyzed. Microbiological assays, plasma membrane permeabilization, confocal laser scanning microscopy, and scanning electron microscopy evaluated biofilm eradication. Statistical significance was set at P < .05.</p><p><strong>Results: </strong>Spectrophotometry indicated Experimental group outperformed Hoshino 1 group and Negative control group (P < .05), with results similar to Hoshino 5 group (P > .05). Experimental group showed increased membrane damage compared to Hoshino 1 group and Hoshino 5 group. Confocal laser scanning microscopy and scanning electron microscopy revealed fewer microorganisms and no residual biofilm in Experimental group.</p><p><strong>Conclusions: </strong>Substituting minocycline with nitrofurantoin in the paste effectively reduced microorganism counts in root canals system.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploring the Potential of Nitrofurantoin for Infection Control in Regenerative Endodontics: In Vitro Study.\",\"authors\":\"Fabiano Palmeira Gonçalves, Olney Vieira-da-Motta, Pantaleo Scelza Neto, Solange Silva Samarão, Sandro Junio de Oliveira Tavares, Sérgio Henrique Seabra, Gabriel Bonan Taveira, Miriam Fatima Zaccaro Scelza\",\"doi\":\"10.1016/j.joen.2024.12.009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>This study assessed a new antimicrobial paste formulation containing nitrofurantoin for regenerative endodontics against multispecies biofilms.</p><p><strong>Methods: </strong>Four groups of 11 single-root teeth each were tested: Negative control group (brain-heart infusion broth + micro-organisms); Hoshino 1 conventional tri-antibiotic paste group (1:1:1 ratio); Hoshino 5 group (5:5:5 ratio); Experimental group with nitrofurantoin, ciprofloxacin, and metronidazole. 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Exploring the Potential of Nitrofurantoin for Infection Control in Regenerative Endodontics: In Vitro Study.
Introduction: This study assessed a new antimicrobial paste formulation containing nitrofurantoin for regenerative endodontics against multispecies biofilms.
Methods: Four groups of 11 single-root teeth each were tested: Negative control group (brain-heart infusion broth + micro-organisms); Hoshino 1 conventional tri-antibiotic paste group (1:1:1 ratio); Hoshino 5 group (5:5:5 ratio); Experimental group with nitrofurantoin, ciprofloxacin, and metronidazole. Minimum inhibitory concentrations of each antimicrobial were determined. A polymicrobial biofilm (Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans) was created in root canals, and pastes were inserted for 3 minutes with lentulo. After 7 days, samples were sonicated and spectrophotometrically analyzed. Microbiological assays, plasma membrane permeabilization, confocal laser scanning microscopy, and scanning electron microscopy evaluated biofilm eradication. Statistical significance was set at P < .05.
Results: Spectrophotometry indicated Experimental group outperformed Hoshino 1 group and Negative control group (P < .05), with results similar to Hoshino 5 group (P > .05). Experimental group showed increased membrane damage compared to Hoshino 1 group and Hoshino 5 group. Confocal laser scanning microscopy and scanning electron microscopy revealed fewer microorganisms and no residual biofilm in Experimental group.
Conclusions: Substituting minocycline with nitrofurantoin in the paste effectively reduced microorganism counts in root canals system.
期刊介绍：
The Journal of Endodontics, the official journal of the American Association of Endodontists, publishes scientific articles, case reports and comparison studies evaluating materials and methods of pulp conservation and endodontic treatment. Endodontists and general dentists can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation in the one journal that helps them keep pace with rapid changes in this field.
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