{"title":"基于Au NPs/三维分层多孔碳网和Au NPs/Cu9S8纳米笼的三明治型电化学免疫传感器检测甲胎蛋白。","authors":"Qi Shen, Jianjun Ding, Zengsheng Guo, Chenchen Wang, Yuhan Zhang, Cuiping Lin, Yiqiang Sun, Lifeng Hang","doi":"10.1016/j.colsurfb.2024.114471","DOIUrl":null,"url":null,"abstract":"<p><p>Alpha-fetoprotein (AFP), serves as a reliable and vital biomarker for precise diagnosis and effective monitoring of hepatocellular carcinoma, requires precise detection. Herein, a sandwich-structured electrochemical immunosensor was crafted, employing three-dimensional layered porous carbon modified with gold nanoparticles (Au NPs) as the substrate and Au NPs/Cu<sub>9</sub>S<sub>8</sub> as the labeling compound for accurate and sensitive detection of AFP. Due to the effective coordination between the 3D carbon network, Au NPs, and hollow Cu<sub>9</sub>S<sub>8</sub> nanocubes, the sandwich-structured electrochemical immunosensor was able to produce three distinct response signals via various detection techniques, demonstrating a broad linear range (0.0001-400 ng/mL), exceptional sensitivity, and a remarkably low detection limit of 2.63 fg/mL. Moreover, the constructed immunosensor could be used to detect AFP in human serum. This research may offer a novel material framework for developing highly sensitive immunosensors.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"114471"},"PeriodicalIF":5.4000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sandwich-type electrochemical immunosensor based on Au NPs/3D hierarchical porous carbon network and Au NPs/Cu<sub>9</sub>S<sub>8</sub> nanocages for the detection of alpha-fetoprotein.\",\"authors\":\"Qi Shen, Jianjun Ding, Zengsheng Guo, Chenchen Wang, Yuhan Zhang, Cuiping Lin, Yiqiang Sun, Lifeng Hang\",\"doi\":\"10.1016/j.colsurfb.2024.114471\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Alpha-fetoprotein (AFP), serves as a reliable and vital biomarker for precise diagnosis and effective monitoring of hepatocellular carcinoma, requires precise detection. Herein, a sandwich-structured electrochemical immunosensor was crafted, employing three-dimensional layered porous carbon modified with gold nanoparticles (Au NPs) as the substrate and Au NPs/Cu<sub>9</sub>S<sub>8</sub> as the labeling compound for accurate and sensitive detection of AFP. Due to the effective coordination between the 3D carbon network, Au NPs, and hollow Cu<sub>9</sub>S<sub>8</sub> nanocubes, the sandwich-structured electrochemical immunosensor was able to produce three distinct response signals via various detection techniques, demonstrating a broad linear range (0.0001-400 ng/mL), exceptional sensitivity, and a remarkably low detection limit of 2.63 fg/mL. Moreover, the constructed immunosensor could be used to detect AFP in human serum. This research may offer a novel material framework for developing highly sensitive immunosensors.</p>\",\"PeriodicalId\":279,\"journal\":{\"name\":\"Colloids and Surfaces B: Biointerfaces\",\"volume\":\"248 \",\"pages\":\"114471\"},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Colloids and Surfaces B: Biointerfaces\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://doi.org/10.1016/j.colsurfb.2024.114471\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/12/22 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"BIOPHYSICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Colloids and Surfaces B: Biointerfaces","FirstCategoryId":"1","ListUrlMain":"https://doi.org/10.1016/j.colsurfb.2024.114471","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/22 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}
Sandwich-type electrochemical immunosensor based on Au NPs/3D hierarchical porous carbon network and Au NPs/Cu9S8 nanocages for the detection of alpha-fetoprotein.
Alpha-fetoprotein (AFP), serves as a reliable and vital biomarker for precise diagnosis and effective monitoring of hepatocellular carcinoma, requires precise detection. Herein, a sandwich-structured electrochemical immunosensor was crafted, employing three-dimensional layered porous carbon modified with gold nanoparticles (Au NPs) as the substrate and Au NPs/Cu9S8 as the labeling compound for accurate and sensitive detection of AFP. Due to the effective coordination between the 3D carbon network, Au NPs, and hollow Cu9S8 nanocubes, the sandwich-structured electrochemical immunosensor was able to produce three distinct response signals via various detection techniques, demonstrating a broad linear range (0.0001-400 ng/mL), exceptional sensitivity, and a remarkably low detection limit of 2.63 fg/mL. Moreover, the constructed immunosensor could be used to detect AFP in human serum. This research may offer a novel material framework for developing highly sensitive immunosensors.
期刊介绍：
Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields.
Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication.
The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.
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