“候选星形植物原体”与印度雏菊叶分学的关联

IF 0.9 4区农林科学 Q4 PLANT SCIENCES

Australasian Plant Pathology Pub Date : 2024-11-27 DOI:10.1007/s13313-024-01004-3

Shweta Singh, K. Prabha, R. K. Saritha, Chandramani Raj, V. K. Baranwal, Skalzang Youdol

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引用次数: 0

摘要

在印度马哈拉施特拉邦的浦那，观察到冠雏菊花表现出典型的叶状和绿化症状。从对症植株中提取总基因组DNA，扩增16S rRNA区域。采用通用植物原体引物进行巢式PCR扩增，证实了植物原体的存在，经测序进一步证实，该植物原体与候选植物原体（Candidatus phytoplasma asteris） 16SrI-B的同源性为100%。对F2n/R2区16S rRNA基因序列（1.2 kb）进行虚拟RFLP分析，与16SrI-B亚群植原体相似系数为1.00。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Association of ‘Candidatus Phytoplasma asteris’ with phyllody of Crown Daisy in India

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Association of ‘Candidatus Phytoplasma asteris’ with phyllody of Crown Daisy in India

The Crown Daisy flowers showing typical phyllody and virescence symptoms were observed in Pune, Maharashtra, India. The total genomic DNA was extracted from the symptomatic plants which was used for amplifying the 16S rRNA region. The nested PCR amplification using universal phytoplasma primers confirmed the phytoplasma presence which was further confirmed upon sequencing which revealed 100% identity with ‘Candidatus Phytoplasma asteris’ 16SrI-B phytoplasmas. Further the virtual RFLP analysis of 16S rRNA gene sequence of F2n/R2 region (1.2 kb) showed a similarity coefficient of 1.00 with 16SrI-B subgroup phytoplasma.

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来源期刊

Australasian Plant Pathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Australasian Plant Pathology presents new and significant research in all facets of the field of plant pathology. Dedicated to a worldwide readership, the journal focuses on research in the Australasian region, including Australia, New Zealand and Papua New Guinea, as well as the Indian, Pacific regions. Australasian Plant Pathology is the official journal of the Australasian Plant Pathology Society.