三功能脂类衍生物：PE的线粒体相互作用组

IF 4.2 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2024-12-26 DOI:10.1039/D4CC03599B

Alix Thomas, Rainer Müller, Scotland Farley, Ana Kojic, Frank Stein, Per Haberkant and Carsten Schultz

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引用次数: 0

摘要

磷脂酰乙醇胺（PE）是高等真核生物中普遍存在的脂类物质，主要存在于线粒体中。在这里，我们合成了一种多功能PE衍生物(1)，旨在通过光交联和随后的PE蛋白偶联物的分离和蛋白质组学分析来鉴定完整细胞中的PE结合蛋白。由于其芳香笼化基团，1最初集中在核周膜上。打开后，观察到线粒体的快速易位。因此，该工具对于跟踪PE位置和确定PE交互组非常有用。相比之下，三功能磷脂酸（PA）衍生物代谢迅速，因此在使用上受到更多限制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Trifunctional lipid derivatives: PE's mitochondrial interactome†

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Trifunctional lipid derivatives: PE's mitochondrial interactome†

Phosphatidylethanolamine (PE) is a ubiquitous lipid species in higher eukaryotes. Here, we synthesized a multifunctionalized PE derivative (1) designed to identify PE-binding proteins in intact cells through photo-crosslinking and subsequent isolation and proteomic analysis of the PE–protein conjugates. We show that the tool is also useful for tracking PE translocation to mitochondria after uncaging. A trifunctional phosphatidic acid derivative was rapidly metabolized and is of more limited use.

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来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.