在莱茵衣藻(Chlamydomonas reinhardtii)中插入诱变AIDA或CYP720B1可获得铜(II)耐受性并增加生物量

IF 12.2 1区 环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL
Ya Li, Lin Jiang, Chuang Xu, Suhui Wang, Yunyao Qian, Yiqiong Wu, Chenyu Miao, Zhouzhou Dong, Liang Wang
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引用次数: 0

摘要

工业和农业环境中铜(Cu)的广泛使用导致过量的铜在水生生态系统中积累,对生物健康构成威胁。微藻生物修复技术已成为缓解风险的一种流行且前景广阔的解决方案。然而,微藻进行重金属生物修复所涉及的遗传基础和工程策略仍未得到充分阐明。在这项研究中,通过筛选莱茵衣藻(C. reinhardtii)突变体文库获得的两个突变体被确定为 AIDA(Cre12.g487450)和 CYP720B1(Cre10.g426700)基因的插入诱变。有趣的是,这两个突变体的细胞大小和纤毛长度都有所下降,但细胞生长率却有所提高。在 Cu(II)胁迫下,AIDA 和 CYP720B1 突变体对 Cu(II)表现出剂量依赖性耐受性,从而增加了生物量并改善了细胞形态。此外,对抗氧化系统的分析表明,Cu(II)耐受性的提高与对 Cu(II)胁迫的低水平响应策略有关。透射电子显微镜图像还显示,这两种突变体中与胁迫相关的细胞器(淀粉粒、酸性钙化体和质粒)有所增加。考虑到这两个突变体对铜(II)的耐受性和生物量都很好,我们的研究结果为进一步遗传改造和性能挖掘提供了潜在的微藻菌株,从而通过提高生物量来改善水生铜(II)的生物修复。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Insertional mutagenesis of AIDA or CYP720B1 in the green alga Chlamydomonas reinhardtii confers copper(II) tolerance and increased biomass

Insertional mutagenesis of AIDA or CYP720B1 in the green alga Chlamydomonas reinhardtii confers copper(II) tolerance and increased biomass
The widespread use of copper (Cu) in industrial and agricultural settings leads to the accumulation of excess Cu within aquatic ecosystems, posing a threat to organism health. Microalgal bioremediation has emerged as a popular and promising solution to mitigate the risks. Nevertheless, the genetic underpinnings and engineering tactics involved in heavy metal bioremediation by microalgae remain inadequately elucidated. In this study, two mutants obtained from screening a Chlamydomonas reinhardtii (C. reinhardtii) mutant library were identified as insertional mutagenesis in the AIDA (Cre12.g487450) and CYP720B1 (Cre10.g426700) genes. Interestingly, these two mutants exhibited decreased cell size and ciliary length but increased cell growth rates. Under Cu(II) stress, the AIDA and CYP720B1 mutants presented dose-dependent tolerance to Cu(II), resulting in increased biomass and improved cellular morphology. Furthermore, the analysis for the antioxidant system suggested that increased Cu(II) tolerance was associated with a low-level response strategy to Cu(II) stress. Transmission electron microscopy images also revealed increased stress-related organelles (starch granules, acidocalcisomes, and plastoglobules) in these two mutants. Considering the excellent Cu(II) tolerance and biomass of these two mutants, our findings provide potential microalgal strains for further genetic modifications and performance mining to improve aquatic Cu(II) bioremediation through biomass enhancement.
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来源期刊
Journal of Hazardous Materials
Journal of Hazardous Materials 工程技术-工程:环境
CiteScore
25.40
自引率
5.90%
发文量
3059
审稿时长
58 days
期刊介绍: The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.
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