抗坏血酸纳米递送系统药用植物细胞外囊泡的分离与表征。

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2024-12-23 DOI:10.1080/02652048.2024.2443430

Zainab Muhammad, Suleiman A Muhammad, Abdullahi Y Abbas, Mohammed Achor, Samson A Adeyemi, Yahya E Choonara, Yusuf Saidu, Lawal S Bilbis

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引用次数: 0

摘要

目的：植物源性细胞外囊泡（EVs）是一种用于药物传递的天然纳米囊泡。本研究从药用植物中分离和表征了电动汽车作为运载工具。方法：采用沉淀法分离，DLS、SEM、TEM进行表征。对抗坏血酸ev的包封率（EE）和抗氧化活性进行了评价。结果：大蒜、姜黄和生姜每重量样品的冻干囊泡总得率分别为6.0、8.6和9.2 mg/g。大蒜源性电动汽车、姜源性电动汽车和姜黄源性电动汽车的平均尺寸分别为101.0±6.7 nm、226.4±62.2 nm和90.7±2.5 nm。电动汽车的zeta电位在-33.2±10.9 ~ -28.8±8.43 mV之间。通过扫描电镜（SEM）和透射电镜（TEM）对纳米囊泡的球形形貌进行了表征。电动汽车的EE在78.1±2.8% ~ 87.2±1.4%之间。结论：总体而言，负载AA的ev抗氧化能力优于游离AA。本研究为这些药用植物是开发纳米治疗药物的丰富资源提供了证据。

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Isolation and characterization of medicinal plant-based extracellular vesicles as nano delivery systems for ascorbic acid.

Aim: Plant-derived extracellular vesicles (EVs) are natural nanovesicles for drug delivery. This study isolated and characterised EVs from medicinal plants as delivery vehicles.

Methods: Precipitation method was employed for the isolation and characterised using DLS, SEM, and TEM. The encapsulation efficiency (EE) and antioxidant activity of ascorbic acid (AA)-EVs were evaluated.

Results: The total yields of lyophilised vesicles per weight of the sample were 6.0, 8.6 and 9.2 mg/g for garlic, turmeric and ginger, respectively. Mean size of garlic-derived EVs, ginger-derived EVs, and turmeric-derived EVs were 101.0 ± 6.7, 226.4 ± 62.2 and 90.7 ± 2.5 nm, respectively. The zeta potential of the EVs was between -33.2 ± 10.9 and -28.8 ± 8.43 mV. Spherical morphology of the nanovesicles was confirmed by SEM and TEM. The EE of the EVs was between 78.1 ± 2.8% and 87.2 ± 1.4%.

Conclusion: Overall, the antioxidant activity of AA-loaded EVs was better compared to free AA. This study provides evidence that these medicinal plants are rich sources for developing nanotherapeutics.

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.