日本产β-内酰胺酶的大肠杆菌ST95高危克隆KLUC-3的出现及遗传特征

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES

Journal of global antimicrobial resistance Pub Date : 2024-12-21 DOI:10.1016/j.jgar.2024.12.012

Wataru Hayashi , Shizuo Kayama , Liansheng Yu , Chiemi Tokushige , Makiko Yuki , Yo Sugawara , Koji Yahara , Motoyuki Sugai

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引用次数: 0

摘要

目的：KLUC β-内酰胺酶（KLUC β-内酰胺酶）是一种次要的延伸谱β-内酰胺酶（ESBL），衍生自克柳甘蓝中染色体编码的头孢噻肟酶。本研究旨在表征产生kucc -3的大肠杆菌和携带blakucc -3的质粒的遗传背景，并评估医院传播。方法：在2019年和2020年进行的全国基因组监测中，从日本一家医院的两名儿科住院患者中分离出产klucc -3的大肠杆菌菌株（jbeach19 -0093和jbeach19 -0210）。使用HiSeq X 5和GridION进行短读和长读测序分析，以确定完整的基因组序列。结果：jbeach -19-0093和jbeach -19-0210属于b2 - 01:K1:H7-ST95-fimH41全球高危克隆，携带肠外致病性和尿路致病性大肠杆菌相关毒力基因。单核苷酸多态性分析显示菌株间具有高度同源性（13个snp），可能存在医院传播。blaKLUC-3基因位于100kb的IncB/O/K/Z质粒上，两侧有ISEcp1和Δorf477，序列完全一致。对比分析显示，isecp1介导blaKLUC-3转位至IncB/O/K/Z质粒；完整的质粒序列与大肠杆菌ST131分离株含有blaCTX-M-和blacmy -2的质粒高度相似。在GenBank数据库中，26种肠杆菌携带blaKLUC-1至blaKLUC-7，特别是在亚洲。其中blaKLUC-orf477/Δorf477基因组结构保守的菌株有23株。在13种肠杆菌中，除低温克雷森菌外，ISEcp1插入blaKLUC上游，10株在质粒上携带blaKLUC。结论：这是中国境外首例KLUC-3产体医院传播病例。在毒性大流行谱系ST95中出现blaKLUC-3是一个公共卫生问题，强调需要进一步调查以防止其潜在传播。

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Emergence and genetic characterization of KLUC-3 extended-spectrum β-lactamase-producing Escherichia coli ST95 High-Risk clone causing nosocomial infection in Japan

Objectives

KLUC β-lactamase is a minor extended-spectrum β-lactamase (ESBL) derived from chromosome-encoded cefotaximase in Kluyvera cryocrescens. This study aimed to characterize the genetic context of KLUC-3-producing Escherichia coli and bla_KLUC-3-harboring plasmids and assess nosocomial transmission.

Methods

In a national genomic surveillance conducted in 2019 and 2020, KLUC-3-producing E. coli strains (JBEAACH-19-0093 and JBEAACH-19-0210) were recovered from two pediatric inpatients in a Japanese hospital. Short- and long-read sequencing analyses using the HiSeq X Five and GridION were performed to determine the complete genome sequences.

Results

JBEAACH-19-0093 and JBEAACH-19-0210 belong to the B2-O1:K1:H7-ST95-fimH41 global high-risk clones and carry virulence genes related to extraintestinal pathogenic and uropathogenic E. coli. Single nucleotide polymorphism analysis showed high homology (13 SNPs) between the strains, suggesting nosocomial transmission. The bla_KLUC-3 gene was flanked by ISEcp1 and Δorf477 on 100-kb IncB/O/K/Z plasmids with a complete sequence identity. Comparative analysis revealed ISEcp1-mediated transposition of bla_KLUC-3 into the IncB/O/K/Z plasmid; the complete plasmid sequence was highly similar to bla_CTX-M- and bla_CMY-2-harboring plasmids from E. coli ST131 isolates. In GenBank database, 26 Enterobacterales harbored bla_KLUC-1 to bla_KLUC-7, particularly in Asia. Among them, the genomic structure of bla_KLUC-orf477/Δorf477 is conserved in 23 strains. In 13 Enterobacterales, except K. cryocrescens, ISEcp1 was inserted upstream of bla_KLUC and 10 strains carried bla_KLUC on the plasmids.

Conclusion

This is the first case of nosocomial transmission of KLUC-3 producers outside China. The bla_KLUC-3 emergence in the virulent pandemic lineage ST95 is a public health problem highlighting the need for further investigations to prevent its potential dissemination.

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来源期刊

Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

8.70

自引率

2.20%

发文量

285

审稿时长

34 weeks

期刊介绍： The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.