{"title":"d -氨基酸代谢酶缺乏对daspo基因敲除小鼠肿瘤发展-弥漫性大b细胞淋巴瘤发病及基因表达的影响","authors":"Yusuke Nakade, Yasunori Iwata, Kenichi Harada, Yasuharu Sato, Masashi Mita, Kenji Hamase, Ryuichi Konno, Mayo Hayashi, Taku Kobayashi, Yuta Yamamura, Tadashi Toyama, Atsushi Tajima, Takashi Wada","doi":"10.1007/s00726-024-03426-1","DOIUrl":null,"url":null,"abstract":"<div><p>The relationship between D-AA metabolic enzymes and cancer development remains unclear. We aimed to investigate this relationship using mice deficient in D-AA-related metabolic enzymes. We examined mice lacking these enzymes for approximately 900 days and the effects of altered D-AA metabolism on cancer development based on lifespan, pathological findings, and gene expression. The lifespan of female <i>DASPO</i> -knockout (<i>DASPO</i><sup><i>−/−</i></sup>) mice was shorter than that of the other group mice; furthermore, these mice showed tumor-like masses in the liver, spleen, and small intestine. A pathological diagnosis of diffuse large B-cell lymphoma (DLBCL) was made. RNA sequencing of the liver samples showed specific alterations in the expression of 71 genes in <i>DASPO</i><sup><i>−/−</i></sup> mice compared with that in wild-type B6 mice; <i>RGS 1</i>, <i>MTSS1</i>, and <i>SMARCD 1</i> were identified as DLBCL-related genes. Patients with DLBCL exhibiting low <i>DASPO</i> expression demonstrated a shorter survival period than those showing high expression. However, the role of <i>DASPO</i> in DLBCL development is unclear. Therefore, future research should focus on B cells. <i>DASPO</i> may serve as novel biomarkers and therapeutic targets in cancer.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"57 1","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00726-024-03426-1.pdf","citationCount":"0","resultStr":"{\"title\":\"Effect of D-amino acid metabolic enzyme deficiency on cancer development—diffuse large B-cell lymphoma onset and gene expression analyses in DASPO-knockout mice\",\"authors\":\"Yusuke Nakade, Yasunori Iwata, Kenichi Harada, Yasuharu Sato, Masashi Mita, Kenji Hamase, Ryuichi Konno, Mayo Hayashi, Taku Kobayashi, Yuta Yamamura, Tadashi Toyama, Atsushi Tajima, Takashi Wada\",\"doi\":\"10.1007/s00726-024-03426-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The relationship between D-AA metabolic enzymes and cancer development remains unclear. We aimed to investigate this relationship using mice deficient in D-AA-related metabolic enzymes. We examined mice lacking these enzymes for approximately 900 days and the effects of altered D-AA metabolism on cancer development based on lifespan, pathological findings, and gene expression. The lifespan of female <i>DASPO</i> -knockout (<i>DASPO</i><sup><i>−/−</i></sup>) mice was shorter than that of the other group mice; furthermore, these mice showed tumor-like masses in the liver, spleen, and small intestine. A pathological diagnosis of diffuse large B-cell lymphoma (DLBCL) was made. RNA sequencing of the liver samples showed specific alterations in the expression of 71 genes in <i>DASPO</i><sup><i>−/−</i></sup> mice compared with that in wild-type B6 mice; <i>RGS 1</i>, <i>MTSS1</i>, and <i>SMARCD 1</i> were identified as DLBCL-related genes. Patients with DLBCL exhibiting low <i>DASPO</i> expression demonstrated a shorter survival period than those showing high expression. However, the role of <i>DASPO</i> in DLBCL development is unclear. Therefore, future research should focus on B cells. <i>DASPO</i> may serve as novel biomarkers and therapeutic targets in cancer.</p><h3>Graphical abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":7810,\"journal\":{\"name\":\"Amino Acids\",\"volume\":\"57 1\",\"pages\":\"\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2024-12-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://link.springer.com/content/pdf/10.1007/s00726-024-03426-1.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Amino Acids\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00726-024-03426-1\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Amino Acids","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s00726-024-03426-1","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Effect of D-amino acid metabolic enzyme deficiency on cancer development—diffuse large B-cell lymphoma onset and gene expression analyses in DASPO-knockout mice
The relationship between D-AA metabolic enzymes and cancer development remains unclear. We aimed to investigate this relationship using mice deficient in D-AA-related metabolic enzymes. We examined mice lacking these enzymes for approximately 900 days and the effects of altered D-AA metabolism on cancer development based on lifespan, pathological findings, and gene expression. The lifespan of female DASPO -knockout (DASPO−/−) mice was shorter than that of the other group mice; furthermore, these mice showed tumor-like masses in the liver, spleen, and small intestine. A pathological diagnosis of diffuse large B-cell lymphoma (DLBCL) was made. RNA sequencing of the liver samples showed specific alterations in the expression of 71 genes in DASPO−/− mice compared with that in wild-type B6 mice; RGS 1, MTSS1, and SMARCD 1 were identified as DLBCL-related genes. Patients with DLBCL exhibiting low DASPO expression demonstrated a shorter survival period than those showing high expression. However, the role of DASPO in DLBCL development is unclear. Therefore, future research should focus on B cells. DASPO may serve as novel biomarkers and therapeutic targets in cancer.
期刊介绍：
Amino Acids publishes contributions from all fields of amino acid and protein research: analysis, separation, synthesis, biosynthesis, cross linking amino acids, racemization/enantiomers, modification of amino acids as phosphorylation, methylation, acetylation, glycosylation and nonenzymatic glycosylation, new roles for amino acids in physiology and pathophysiology, biology, amino acid analogues and derivatives, polyamines, radiated amino acids, peptides, stable isotopes and isotopes of amino acids. Applications in medicine, food chemistry, nutrition, gastroenterology, nephrology, neurochemistry, pharmacology, excitatory amino acids are just some of the topics covered. Fields of interest include: Biochemistry, food chemistry, nutrition, neurology, psychiatry, pharmacology, nephrology, gastroenterology, microbiology
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