某些毒物的杀螺评估：对masssylaea vermiculata蜗牛生化改变和电泳蛋白模式的影响（O. F. m ller, 1774）。

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2025-01-01 DOI:10.1016/j.etap.2024.104619

Sahar I.M. Abd El-Wahed , Hesham A.M. Ibrahim

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引用次数: 0

摘要

马螺是埃及重要的腹足类害虫，对农业构成威胁。由于人们对传统农药的担忧日益增加，寻找危害较小的有效替代品势在必行。采用叶片浸渍法测定了阿维菌素、甲氧虫酰肼和spinosad的体外杀螺活性，以及它们的LC50对生化和蛋白电泳的影响。结果表明，这些化合物对阿维菌素、甲氧虫腈和spinosad的LC50分别为0.21、0.63和0.65mg/l。生化试验结果表明，与对照相比，处理后的钉螺的天冬氨酸、丙氨酸转氨酶和碱性磷酸酶活性增加，总蛋白含量降低。治疗10 d后，最有效的复方阿维菌素分别为195.36、105.82、276.76和2.49，对照组分别为88.00、47.67、124.67和5.52。蛋白质电泳显示蛋白质条带的变化。因此，这些化合物在综合控制方案中是有效的。

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Molluscicidal assessment of certain toxicants: Impact on biochemical alterations and electrophoretic protein patterns in Massylaea vermiculata (O. F. Müller, 1774) snails

Massylaea vermiculata snails are a significant gastropod pest in Egypt, threatening agriculture. Due to increasing concerns about conventional pesticides, it is imperative to find effective alternatives that are less harmful. We assessed the molluscicidal activity of abamectin, methoxyfenozide, and spinosad using the leaf-dipping method in vitro and the effect of LC₅₀ of these compounds on biochemical aspects and protein electrophoresis. Results showed that these compounds exhibited molluscicidal activity, with LC₅₀ values of 0.21, 0.63, and 0.65 mg/l for abamectin, methoxyfenozide and spinosad, respectively. Biochemical assays on treated snails showed increased aspartate and alanine aminotransferase and alkaline phosphatase activities and reduced total protein compared to controls. For the most effective compound (abamectin), these values were 195.36, 105.82, 276.76, and 2.49, compared to control values of 88.00, 47.67, 124.67, and 5.52, after 10 days post-treatment. Protein electrophoresis revealed variations in protein bands. Thus, these compounds can be effective within integrated control programs.

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.