Anika Retzmann, Kerri A Miller, Fwziah Ali Abdalali Mohamed, Michael E Wieser
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引用次数: 0
摘要
采用prepFAST MC™系统（Elemental Scientific）、DGA树脂（TrisKem International）和TK201树脂（TrisKem International），为后续Zn同位素分析设计了一种全自动双柱纯化生物样品Zn的程序。所开发的程序每天可实现20个样品的无人处理，其特点是低且可重复的空白（50次），高锌收率100.1%±5.3% (2 SD, N = 22)，对生物样品（骨骼，肝脏，头发，血液）的基质变化具有很强的稳健性。此外，使用MC-ICP-MS进行Zn同位素分析没有发现明显的柱上分馏。NIST SRM 1400（0.67‰±0.07‰，U, k = 2）、NIST SRM 1486（0.91‰±0.06‰，U, k = 2）、NIST SRM 1577c（- 0.45‰±0.05‰，U, k = 2）、ERM-DB001（- 0.35‰±0.05‰，U, k = 2）、GBW09101（- 0.32‰±0.08‰，U, k = 2）和SeroNorm全血L-3（-0.15‰±0.05‰，U, k = 2）的δ66Zn/64ZnIRMM测量值与已发表的值一致。开发的程序使Zn（一种具有分析挑战性的同位素系统）更容易获得、可行和可靠，适用于更广泛的用户，同时实现高样品通量。
Reliable and precise Zn isotopic analysis of biological matrices using a fully automated dual-column purification procedure.
A fully automated dual-column purification procedure for Zn from biological samples, designed for subsequent Zn isotopic analysis, is presented that utilizes the prepFAST MC™ system (Elemental Scientific), DGA resin (TrisKem International), and TK201 resin (TrisKem International). The procedure developed enables the unattended processing of 20 samples per day and is characterized by low and reproduceable blanks (< 1.5 ng), no carry-over or memory effect, high reusability (> 50 times), high Zn yields 100.1% ± 5.3% (2 SD, N = 22), and strong robustness to matrix variations across biological samples (bone, liver, hair, blood). Additionally, Zn isotopic analysis using MC-ICP-MS showed no significant on-column fractionation. The measured δ66Zn/64ZnIRMM values for NIST SRM 1400 (0.67‰ ± 0.07‰, U, k = 2), NIST SRM 1486 (0.91‰ ± 0.06‰, U, k = 2), NIST SRM 1577c (- 0.45‰ ± 0.05‰, U, k = 2), ERM-DB001 (- 0.35‰ ± 0.05‰, U, k = 2), GBW09101 (- 0.32‰ ± 0.08‰, U, k = 2), and SeroNorm whole blood L-3 (-0.15 ‰ ± 0.05 ‰, U, k = 2) are consistent with published values. The procedure developed makes Zn, an analytically challenging isotope system, more accessible, feasible, and reliable for a broader range of users while enabling high sample throughput.
期刊介绍：
Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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