超声辅助深共晶溶剂三相萃取法提取西洋参多糖：工艺优化、结构表征及抗溃疡性结肠炎研究。

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2025-01-01 DOI:10.1016/j.ultsonch.2024.107206

Zhongnan Wu , Chong Li , Junhao Li , Tanggan Wang , Meifeng Li , Leyi Zhao , Huimei Ye , Jiaheng Chen , Jiajia Zan , Lijun Song , Qian Zhang , Shaojie Zhang

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引用次数: 0

摘要

三相分割法是分离生物活性多糖的有效方法，但丁醇对环境的影响限制了其应用。深共晶溶剂可以作为一种绿色的、可回收的丁醇替代品。本研究采用超声辅助DES三相分割（UA-TPP-DES）系统提取纯化西洋参多糖。优化后的DES-4由月桂酸和壬酸（摩尔比为1:1）组成，在特定条件下提取率为35.28%，可循环使用5次，收率损失最小。AGP-DES-4分子量分布广泛（2.48-174.64 kDa），主要由甘露糖、葡萄糖、半乳糖和阿拉伯糖组成。体内果蝇模型表明，AGP-DES-4通过调节肠上皮细胞（IECs）和肠干细胞（ISCs）的增殖和分化，提高UC蝇的生存能力，增强肠道屏障功能。我们的研究结果突出了AGP-DES-4提取方法的有效性及其治疗UC的潜在治疗价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Extraction of American ginseng polysaccharide by ultrasound-assisted deep eutectic solvents-based three-phase partitioning: Process optimization, structural characterization, and anti-ulcerative colitis study

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Three-phase partitioning (TPP) is promising for isolating bioactive polysaccharides, but t-butanol’s environmental impact limits its application. Deep eutectic solvents can serve as a green and recyclable alternative to t-butanol. This study introduces an ultrasonic-assisted DES three-phase partitioning (UA-TPP-DES) system to extract and purify American ginseng polysaccharides (AGPs). The optimized DES-4, composed of lauric acid and nonanoic acid (molar ratio = 1:1), achieved a 35.28 % extraction yield under specific conditions and can be recycled five times with minimal yield loss. AGP-DES-4 has a broad molecular weight distribution (2.48–174.64 kDa) and mainly consists of mannose, glucose, galactose, and arabinose. In vivo Drosophila models show that AGP-DES-4 improves UC fly survival and enhances intestinal barrier function by regulating the proliferation and differentiation of intestinal epithelial cells (IECs) and intestinal stem cells (ISCs). Our results highlight the effectiveness of the AGP-DES-4 extraction method and its potential therapeutic value for treating UC.

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来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.