嗜酸乳杆菌膜泡减轻帕金森病大鼠肠道炎症，增加黑质5-羟色胺。

IF 4.7 3区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Archives of Medical Research Pub Date : 2024-12-19 DOI:10.1016/j.arcmed.2024.103143

Khadijeh Sheikh, Javad Arasteh, Maryam Tajabadi Ebrahimi, Ardeshir Hesampour

{"title":"嗜酸乳杆菌膜泡减轻帕金森病大鼠肠道炎症，增加黑质5-羟色胺。","authors":"Khadijeh Sheikh, Javad Arasteh, Maryam Tajabadi Ebrahimi, Ardeshir Hesampour","doi":"10.1016/j.arcmed.2024.103143","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>This study aimed to investigate the role of membrane vesicles (MVs) from the probiotic Lactobacillus acidophilus in reducing intestinal inflammation and increasing 5-hydroxytryptamine (5-HT) and tyrosine hydroxylase (TH) in the substantia nigra in the 6-hydroxydopamine (6-OHDA) rat model of Parkinson's disease (PD).</div></div><div><h3>Methods</h3><div>Twenty healthy male Wistar rats were randomly assigned to four groups (<em>n</em> = 5 per group), including a) control, b) 6-OHDA, c) 6-OHDA+MV, and d) sham groups. PD was induced by bilateral injection of 6-OHDA. Rats in the 6-OHDA+MV group received MV equivalent to 1 × 10<sup>7</sup> colony-forming units (CFU)/mL 3 d/wk by oral gavage for 4 wk. At the end of 4 wk, all rats were sacrificed; the brain and small intestine were removed for cellular and molecular analysis.</div></div><div><h3>Results</h3><div>The induction of PD by 6-OHDA induced a remarkable decrease in beam-walking (<em>p</em> < 0.0001). In addition, the expression of protein and genes (receptor) of 5-HT (r-5-HT1A) decreased, and that of protein and gene (receptor; GABBR1) of GABA increased in the PD group <em>(p</em> < 0.05 compared with the healthy control group), while MV gavage of 6-OHDA-injected rats controlled these factors in the substantia nigra. In the intestinal tissue, the expression of TLR-4 and α-synuclein gene was significantly increased in the 6-OHDA group compared to the control group (<em>p</em> < 0.0001).</div></div><div><h3>Conclusion</h3><div>MVs might act as potential beneficial tools to reduce intestinal inflammation, control neurological damage associated with PD, and increase 5-HT neurotransmitters. It seems that MVs from L. acidophilus may have therapeutic potential in Parkinson's neurological disorder by controlling the gut-brain axis.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103143"},"PeriodicalIF":4.7000,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Membrane vesicles from lactobacillus acidophilus reduce intestinal inflammation and increase 5-HT in the substantia nigra of rats with parkinson's disease\",\"authors\":\"Khadijeh Sheikh, Javad Arasteh, Maryam Tajabadi Ebrahimi, Ardeshir Hesampour\",\"doi\":\"10.1016/j.arcmed.2024.103143\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>This study aimed to investigate the role of membrane vesicles (MVs) from the probiotic Lactobacillus acidophilus in reducing intestinal inflammation and increasing 5-hydroxytryptamine (5-HT) and tyrosine hydroxylase (TH) in the substantia nigra in the 6-hydroxydopamine (6-OHDA) rat model of Parkinson's disease (PD).</div></div><div><h3>Methods</h3><div>Twenty healthy male Wistar rats were randomly assigned to four groups (<em>n</em> = 5 per group), including a) control, b) 6-OHDA, c) 6-OHDA+MV, and d) sham groups. PD was induced by bilateral injection of 6-OHDA. Rats in the 6-OHDA+MV group received MV equivalent to 1 × 10<sup>7</sup> colony-forming units (CFU)/mL 3 d/wk by oral gavage for 4 wk. At the end of 4 wk, all rats were sacrificed; the brain and small intestine were removed for cellular and molecular analysis.</div></div><div><h3>Results</h3><div>The induction of PD by 6-OHDA induced a remarkable decrease in beam-walking (<em>p</em> < 0.0001). In addition, the expression of protein and genes (receptor) of 5-HT (r-5-HT1A) decreased, and that of protein and gene (receptor; GABBR1) of GABA increased in the PD group <em>(p</em> < 0.05 compared with the healthy control group), while MV gavage of 6-OHDA-injected rats controlled these factors in the substantia nigra. In the intestinal tissue, the expression of TLR-4 and α-synuclein gene was significantly increased in the 6-OHDA group compared to the control group (<em>p</em> < 0.0001).</div></div><div><h3>Conclusion</h3><div>MVs might act as potential beneficial tools to reduce intestinal inflammation, control neurological damage associated with PD, and increase 5-HT neurotransmitters. It seems that MVs from L. acidophilus may have therapeutic potential in Parkinson's neurological disorder by controlling the gut-brain axis.</div></div>\",\"PeriodicalId\":8318,\"journal\":{\"name\":\"Archives of Medical Research\",\"volume\":\"56 3\",\"pages\":\"Article 103143\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2024-12-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of Medical Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0188440924001942\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Medical Research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0188440924001942","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

摘要

背景：本研究旨在探讨益生菌嗜酸乳杆菌膜囊泡（MVs）在帕金森病（PD）大鼠6-羟多巴胺（6-OHDA）模型中减轻肠道炎症和增加黑质5-羟色胺（5-HT）和酪氨酸羟化酶（TH）的作用。方法：健康雄性Wistar大鼠20只，随机分为a)对照组、b) 6-OHDA组、c) 6-OHDA+MV组、d)假手术组，每组n = 5只。双侧注射6-OHDA诱导PD。6-OHDA+MV组给予相当于1 × 107菌落形成单位(CFU)/mL的MV， 3 d/周灌胃，持续4周。4周结束时，处死所有大鼠；切除脑和小肠进行细胞和分子分析。结果：6-OHDA诱导PD可显著降低束行（p < 0.0001）。5-HT （r-5-HT1A）蛋白和基因（受体）表达降低；PD组GABA的GABBR1含量升高（与健康对照组比较p < 0.05），而注射6-羟色胺的大鼠灌胃MV可控制黑质中这些因子的表达。在肠组织中，6-OHDA组TLR-4和α-synuclein基因的表达较对照组显著升高（p < 0.0001）。结论：MVs可能作为潜在的有益工具，减少肠道炎症，控制PD相关的神经损伤，增加5-HT神经递质。嗜酸乳杆菌的MVs可能通过控制肠-脑轴而具有治疗帕金森神经系统疾病的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Membrane vesicles from lactobacillus acidophilus reduce intestinal inflammation and increase 5-HT in the substantia nigra of rats with parkinson's disease

Background

This study aimed to investigate the role of membrane vesicles (MVs) from the probiotic Lactobacillus acidophilus in reducing intestinal inflammation and increasing 5-hydroxytryptamine (5-HT) and tyrosine hydroxylase (TH) in the substantia nigra in the 6-hydroxydopamine (6-OHDA) rat model of Parkinson's disease (PD).

Methods

Twenty healthy male Wistar rats were randomly assigned to four groups (n = 5 per group), including a) control, b) 6-OHDA, c) 6-OHDA+MV, and d) sham groups. PD was induced by bilateral injection of 6-OHDA. Rats in the 6-OHDA+MV group received MV equivalent to 1 × 10⁷ colony-forming units (CFU)/mL 3 d/wk by oral gavage for 4 wk. At the end of 4 wk, all rats were sacrificed; the brain and small intestine were removed for cellular and molecular analysis.

Results

The induction of PD by 6-OHDA induced a remarkable decrease in beam-walking (p < 0.0001). In addition, the expression of protein and genes (receptor) of 5-HT (r-5-HT1A) decreased, and that of protein and gene (receptor; GABBR1) of GABA increased in the PD group (p < 0.05 compared with the healthy control group), while MV gavage of 6-OHDA-injected rats controlled these factors in the substantia nigra. In the intestinal tissue, the expression of TLR-4 and α-synuclein gene was significantly increased in the 6-OHDA group compared to the control group (p < 0.0001).

Conclusion

MVs might act as potential beneficial tools to reduce intestinal inflammation, control neurological damage associated with PD, and increase 5-HT neurotransmitters. It seems that MVs from L. acidophilus may have therapeutic potential in Parkinson's neurological disorder by controlling the gut-brain axis.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Archives of Medical Research 医学-医学：研究与实验

CiteScore

12.50

自引率

0.00%

发文量

审稿时长

28 days

期刊介绍： Archives of Medical Research serves as a platform for publishing original peer-reviewed medical research, aiming to bridge gaps created by medical specialization. The journal covers three main categories - biomedical, clinical, and epidemiological contributions, along with review articles and preliminary communications. With an international scope, it presents the study of diseases from diverse perspectives, offering the medical community original investigations ranging from molecular biology to clinical epidemiology in a single publication.