在体外细胞培养模型中从吞噬MRSA的肺泡巨噬细胞中分离细胞外囊泡的方法。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2024-12-20 DOI:10.1016/j.xpro.2024.103526

Songjie Bai, Ruomeng Hu, Xuehuan Wen, Wenting Zhang, Bingyu Li, Jie Yang, Qing Yu, Xianchang Zeng, Huiqing Xiu, Zhijian Cai, Gensheng Zhang

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引用次数: 0

摘要

细胞外囊泡（EVs）在感染性疾病的生物活性转运中起着至关重要的作用。在这里，我们提出了一种在体外细胞培养模型中从吞噬耐甲氧西林金黄色葡萄球菌（MRSA）的肺泡巨噬细胞（AMs）中分离ev的方案。我们描述了细菌制备的步骤；AMs感染MRSA；以及电动汽车的分离、纯化和表征。该方案为研究由病原体感染的免疫细胞衍生的ev提供了有价值的视角。有关本协议使用和执行的完整细节，请参阅Bai等人1。

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Protocol for isolating extracellular vesicles from alveolar macrophages phagocytosing MRSA in vitro cell culture models.

Extracellular vesicles (EVs) play a crucial role in delivering bioactive cargo in infectious diseases. Here, we present a protocol for isolating EVs from alveolar macrophages (AMs) that phagocytose methicillin-resistant Staphylococcus aureus (MRSA) in vitro cell culture models. We describe steps for bacterial preparation; infection of AMs with MRSA; and isolation, purification, and characterization of EVs. This protocol provides a valuable perspective for studying EVs derived from pathogen-infected immune cells. For complete details on the use and execution of this protocol, please refer to Bai et al.¹.

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