干血斑：一种单滴血检测HBV、HCV、HIV核酸的诊断方法。

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2024-12-17 DOI:10.1016/j.diagmicrobio.2024.116661

Stephen Strunge Nilsson , Jonas Demant , Sara Thønnings , Nina Weis , Henrik Westh , Mette Pinholt

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引用次数: 0

摘要

到2030年根除乙型肝炎（HBV）、丙型肝炎（HCV）和艾滋病毒的全球战略至关重要，因为它们对卫生保健系统的影响和挑战。丙型肝炎是可治愈的，但乙型肝炎和艾滋病毒只有抑制作用，只有乙型肝炎疫苗可用。需要创新的诊断方法，特别是针对注射吸毒者等高危人群。本研究验证了使用Hologic Panther系统检测HBV、HCV和HIV的干血斑（DBS）核酸扩增试验（NAAT）。该方法用于丹麦首都地区PWID人群的筛查。DBS方法具有较高的灵敏度，95%的检出限（LoD）为：HBV 2711 IU/mL， HCV 525 IU/mL, HIV 4022 copies/mL。丹麦83例PWID的筛查显示，活动性HCV感染的患病率为13%，这在传统静脉通路困难的环境中提供了显著的益处。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Dried blood spot: A diagnostic detection method for HBV, HCV and HIV nucleic acid using a single drop of blood

The global strategy to eradicate Hepatitis B (HBV), Hepatitis C (HCV), and HIV by 2030 is critical due to their impact and challenges to healthcare systems. HCV is curable, but HBV and HIV are only suppressible, with a vaccine available solely for HBV. Innovative diagnostic methods are needed, especially for high-risk populations like people who inject drugs (PWID). This study validates a dried blood spot (DBS) nucleic acid amplification test (NAAT) using the Hologic Panther system for detecting HBV, HCV, and HIV. The method was used to screen among PWID in the Capital Region of Denmark. The DBS method demonstrated high sensitivity, with a 95 % limit of detection (LoD) of 2711 IU/mL for HBV, 525 IU/mL for HCV, and 4022 copies/mL for HIV. Screening of 83 PWID in Denmark revealed a 13 % prevalence of active HCV infection, offering significant benefits in settings where traditional venous access is difficult.

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.